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PMID: 8404846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SCAMP 37, a new marker within the general cell surface recycling system.

The EMBO journal ·Vol. 12 ·No. 10 ·1993-10-00 ·Pages 3753-61

Brand SH, Castle JD

Abstract

Secretory carrier membrane proteins (SCAMPs) are widely distributed as components of post-Golgi membranes that function as recycling carriers to the cell surface. In fibroblasts, SCAMPs are concentrated in compartments involved in the endocytosis and recycling of cell surface receptors while in neurons and other cell types having regulated transport pathways, SCAMPs are also components of regulated carriers (synaptic vesicles, secretion granules and transporter vesicles). Their presence in multiple pathways distinguishes them from proteins (e.g. recycling cell surface receptors and synaptic vesicle proteins) which are concentrated in selected pathways. The SCAMPs also do not appear to reside beyond the boundaries of these pathways. This distribution suggests that SCAMPs are general markers of membranes that function in cell surface recycling. The primary sequence of SCAMP 37 reveals a novel polypeptide containing a series of structural motifs, including a calcium binding domain, a leucine zipper and two zinc fingers. The very broad tissue distribution, subcellular localization and sequence analysis all predict that SCAMPs play a fundamental role in cell surface recycling.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biomarkers Blotting, Northern Blotting, Western Brain/metabolism Carrier Proteins/genetics,isolation & purification,metabolism Cell Line Cloning, Molecular DNA Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Golgi Apparatus/metabolism Intracellular Signaling Peptides and Proteins Membrane Proteins/genetics,isolation & purification,metabolism Molecular Sequence Data Organ Specificity RNA, Messenger Rats Repetitive Sequences, Nucleic Acid
Chemicals
Biomarkers Carrier Proteins Intracellular Signaling Peptides and Proteins Membrane Proteins RNA, Messenger Scamp1 protein, rat DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brand S H
Department of Anatomy and Cell Biology, University of Virginia Health Sciences Center, Charlottesville 22908.
Castle J D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-10-00
Pages
3753-61
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413657
Subset
IM
Grants
NIDCR NIH HHS · DE09655 · United States
External Links
PubMed source PMC Full Text View journal details
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