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PMID: 8396582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of Ockelbo virus RNA in skin biopsies by polymerase chain reaction.

Journal of clinical microbiology ·Vol. 31 ·No. 8 ·1993-08-00 ·Pages 2004-9

Hörling J, Vene S, Franzén C, Niklasson B

Abstract

A sensitive assay based on the polymerase chain reaction for the detection of Ockelbo virus RNA was developed. Two primer pairs from the gene coding for the E2 glycoprotein were chosen. By use of a nested strategy for the primers, as few as 1 to 10 PFU could be detected. The amplified products were visualized as bands of appropriate size on ethidium bromide-stained agarose gels. The primer pairs allowed amplification of several Ockelbo and Sindbis virus isolates but discriminated between these and other alphaviruses. Ockelbo virus RNA was detected in 4 of 10 skin biopsy specimens collected during the acute stage of the disease. The identities of the amplified products were confirmed by restriction endonuclease cleavage. Acute- and convalescent-phase sera as well as lymphocytes collected during the convalescent phase were negative by the polymerase chain reaction. No infectious virus could be recovered from any of the specimens.

MeSH Terms
Base Sequence Biopsy Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Viral/analysis Skin/microbiology Togaviridae/genetics,isolation & purification Togaviridae Infections/microbiology
Chemicals
RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hörling J
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Vene S
Franzén C
Niklasson B
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.8.2004-2009.1993
Published
1993-08-00
Pages
2004-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265687
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.31.8.2004-2009.1993 PMC Full Text View journal details
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