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PMID: 8395350 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Three-dimensional visualization of the rotavirus hemagglutinin structure.

Cell ·Vol. 74 ·No. 4 ·1993-08-27 ·Pages 693-701

Shaw AL, Rothnagel R, Chen D, Ramig RF, Chiu W, Prasad BV

Abstract

Three-dimensional structures of a native simian and reassortant rotavirus have been determined by electron cryomicroscopy and computer image processing. The structural features of the native virus confirm that the hemagglutinin spike is a dimer of VP4, substantiated by in vivo radiolabeling studies. Exchange of native VP4 with a bovine strain equivalent results in a poorly infectious reassortant. No VP4 spikes are detected in the three-dimensional reconstruction of the reassortant. The difference map between the two structures reveals a novel large globular domain of VP4 buried within the virion that interacts extensively with the intermediate shell protein, VP6. Our results suggest that assembly of VP4 precedes that of VP7, the major outer shell protein, and that VP4 may play an important role in the receptor recognition and budding process through the rough endoplasmic reticulum during virus maturation.

MeSH Terms
Animals Capsid/biosynthesis,ultrastructure Capsid Proteins Cell Line Computer Graphics Freezing Hemagglutinins, Viral/biosynthesis,ultrastructure Macromolecular Substances Methionine/metabolism Microscopy, Electron/methods Models, Structural Rotavirus/metabolism,ultrastructure
Chemicals
Capsid Proteins Hemagglutinins, Viral Macromolecular Substances VP4 protein, Rotavirus Methionine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shaw A L
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.
Rothnagel R
Chen D
Ramig R F
Chiu W
Prasad B V
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(93)90516-s
Published
1993-08-27
Pages
693-701
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133302
Subset
IM
Grants
NIAID NIH HHS · AI16687 · United States
NIGMS NIH HHS · GM41064 · United States
NCRR NIH HHS · RR02250 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(93)90516-s PMC Full Text View journal details
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