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PMID: 8393010 Published · ppublish English Journal Article

Delayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes.

Journal of bacteriology ·Vol. 175 ·No. 15 ·1993-08-00 ·Pages 4905-6

Prakash-Cheng A, Ryu J

Abstract

Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified host and suggests regulation of restriction activity after conjugal transfer.

Related Genes
MeSH Terms
Conjugation, Genetic/genetics,physiology DNA Restriction Enzymes/genetics Escherichia coli/enzymology,genetics Gene Expression Regulation, Bacterial/genetics,physiology Gene Expression Regulation, Enzymologic/genetics,physiology Genes, Bacterial/genetics,physiology
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prakash-Cheng A
Department of Microbiology, School of Medicine, Loma Linda University, California 92350.
Ryu J
References (12)
12 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1993-08-00
Pages
4905-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204944
Subset
IM
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