Abstract
Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified host and suggests regulation of restriction activity after conjugal transfer.
MeSH Terms
Conjugation, Genetic/genetics,physiology
DNA Restriction Enzymes/genetics
Escherichia coli/enzymology,genetics
Gene Expression Regulation, Bacterial/genetics,physiology
Gene Expression Regulation, Enzymologic/genetics,physiology
Genes, Bacterial/genetics,physiology
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prakash-Cheng A
Department of Microbiology, School of Medicine, Loma Linda University, California 92350.
Ryu J
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