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PMID: 8391061 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pore-forming bacterial toxins potently induce release of nitric oxide in porcine endothelial cells.

The Journal of experimental medicine ·Vol. 178 ·No. 1 ·1993-07-01 ·Pages 337-41

Suttorp N, Fuhrmann M, Tannert-Otto S, Grimminger F, Bhadki S

Abstract

Nitric oxide (NO) is believed to play an important role in sepsis-related hypotension. We examined the effects of two pore-forming bacterial exotoxins, Escherichia coli hemolysin and Staphylococcus aureus alpha-toxin, on NO formation in cultured porcine pulmonary artery endothelial cells. NO was quantified using a difference-spectrophotometric method based on the rapid and stoichiometric reaction of NO with oxyhemoglobin. Endothelial cyclic guanosine monophosphate levels were also monitored. Both exotoxins increased NO synthesis in endothelial cells in a time- and dose-dependent manner to an extent exceeding that observed with the ionophore A23187 or thrombin. The capacity of exotoxins to induce NO formation may be relevant in patients with severe local or systemic bacterial infections.

MeSH Terms
Animals Bacterial Proteins/pharmacology Bacterial Toxins/pharmacology Calcium/metabolism Cells, Cultured Cyclic GMP/analysis Endothelium, Vascular/drug effects,metabolism Escherichia coli Proteins Hemolysin Proteins/pharmacology Nitric Oxide/metabolism Swine
Chemicals
Bacterial Proteins Bacterial Toxins Escherichia coli Proteins Hemolysin Proteins Hlya protein, E coli staphylococcal alpha-toxin Nitric Oxide Cyclic GMP Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Suttorp N
Department of Internal Medicine, Justus-Liebig University, Giessen, Germany.
Fuhrmann M
Tannert-Otto S
Grimminger F
Bhadki S
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.178.1.337
Published
1993-07-01
Pages
337-41
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191086
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.178.1.337 PMC Full Text View journal details
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