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PMID: 8390818 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The Bradyrhizobium japonicum serocluster 123 hyperreiterated DNA region, HRS1, has DNA and amino acid sequence homology to IS1380, an insertion sequence from Acetobacter pasteurianus.

Applied and environmental microbiology ·Vol. 59 ·No. 5 ·1993-05-00 ·Pages 1656-61

Judd AK, Sadowsky MJ

Abstract

We have sequenced and analyzed the hyperreiterated DNA region, HRS1, from Bradyrhizobium japonicum USDA 424. The 2.1-kb HRS1 fragment is closely linked to the B. japonicum common and genotype-specific nodulation genes in serogroup 123 and 127 strains. Southern hybridization analyses indicated that one copy of HRS1 is also located next to the fixRnifA locus in B. japonicum USDA 424. Nucleotide sequence analysis revealed the presence of a 4-bp target site duplication in HRS1 which is identical to a terminal repeat found in the B. japonicum USDA 110 repeated sequence RS alpha. Computer searches of the PIR (Protein Identification Resource) protein data base revealed a high degree of amino acid sequence homology between a putative 329-amino-acid polypeptide from HRS1 and a large polypeptide from IS1380, an insertion sequence from Acetobacter pasteurianus. RNA slot blot hybridizations suggest that transcripts showing homology to HRS1 are constitutively produced in strains USDA 424 (serogroup 127) and USDA 438 (serogroup 123).

MeSH Terms

Acetobacter/genetics Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosome Mapping DNA Transposable Elements DNA, Bacterial/genetics Genetic Variation Molecular Sequence Data Repetitive Sequences, Nucleic Acid Rhizobiaceae/classification,genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Serotyping

Chemicals

Bacterial Proteins DNA Transposable Elements DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Judd A K

Soil Science Department, University of Minnesota, St. Paul 55108.

Sadowsky M J

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21 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.59.5.1656-1661.1993

Published

1993-05-00

Pages

1656-61

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC182133

Subset

Databases

GENBANK

L09226, X62978, X62979, X62980, X62981, X62986, X62987, X62988, X62989, X62990

External Links

PubMed source DOI: 10.1128/aem.59.5.1656-1661.1993 PMC Full Text View journal details

Analysis Services

The Bradyrhizobium japonicum serocluster 123 hyperreiterated DNA region, HRS1, has DNA and amino acid sequence homology to IS1380, an insertion sequence from Acetobacter pasteurianus.

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