Home LiteratureArticle Details

PMID: 8386978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Design of primers for PCR amplification of highly variable genomes.

Computer applications in the biosciences : CABIOS ·Vol. 9 ·No. 2 ·1993-04-00 ·Pages 123-5

Dopazo J, Rodríguez A, Sáiz JC, Sobrino F

Abstract

A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.

MeSH Terms

Aphthovirus/genetics DNA, Viral/analysis Diagnosis, Computer-Assisted Polymerase Chain Reaction/methods Sequence Alignment Software

Chemicals

DNA, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Dopazo J

INIA-Sanidad Animal, Madrid, Spain.

Rodríguez A

Sáiz J C

Sobrino F

Article Info

Journal

Computer applications in the biosciences : CABIOS

Abbr.

Comput Appl Biosci

ISSN

0266-7061

DOI

10.1093/bioinformatics/9.2.123

Published

1993-04-00

Pages

123-5

Language

English

Region

England

NLM ID

8511758

Subset

External Links

PubMed source DOI: 10.1093/bioinformatics/9.2.123

Analysis Services

Design of primers for PCR amplification of highly variable genomes.

Contact

WeChat Official Account