Home LiteratureArticle Details
PMID: 838682 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Comparison of nucleic acid content in populations of free-living and symbiotic Rhizobium meliloti by flow microfluorometry.

Journal of bacteriology ·Vol. 129 ·No. 2 ·1977-02-00 ·Pages 1156-8

Paau AS, Lee D, Cowles JR

Abstract

Populations of symbiotic Rhizobium meliloti extracted from alfalfa nodules were shown by flow microfluorometry to contain a significant number of bacteroids with higher nucleic acid content than the free-living rhizobia. Bacteroids with lower nucleic acid content than the free-living bacteria were not detected in significant quantities in these populations. These results indicate that the incapability of bacteroids to reestablish growth in nutrient media may not be caused by a decrease in nucleic acid content of the symbiotic rhizobia.

MeSH Terms
DNA, Bacterial/analysis Fluorometry/methods Medicago sativa/microbiology Rhizobium/analysis Soil Microbiology Symbiosis
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Paau A S
Lee D
Cowles J R
References (7)
7 references, click to expand
  1. A new multiparameter separator for microscopic particles and biological cells.
    Rev Sci Instrum. 1973 Sep;44(9):1301-10 PMID: 4279087
  2. Some features of the DNA of Rhizobium bacteroids and bacteria.
    Biochim Biophys Acta. 1974 Sep 27;366(1):1-10 PMID: 4417115
  3. Some properties of the ribonucleotide reductase from Rhizobium meliloti.
    Arch Biochem Biophys. 1968 Sep 20;127(1):770-8 PMID: 4880553
  4. ELECTRON MICROSCOPY OF INFECTION THREADS AND BACTERIA IN YOUNG ROOT NODULES OF MEDICAGO SATIVA.
    J Bacteriol. 1963 Jul;86:125-37 PMID: 14051804
  5. Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations.
    J Cell Biol. 1973 Dec;59(3):766-71 PMID: 4128323
  6. The bacterial component of soybean root nodules; changes in respiratory activity, cell dry weight and nucleic acid content with increasing nodule age.
    J Gen Microbiol. 1958 Oct;19(2):312-23 PMID: 13587897
  7. Comparison of DNA Polymerase of Rhizobium meliloti and Alfalfa Bacteroids.
    Plant Physiol. 1975 Oct;56(4):526-8 PMID: 16659337
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.129.2.1156-1158.1977
Published
1977-02-00
Pages
1156-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235060
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.129.2.1156-1158.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2