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PMID: 8370755 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Prospective application of reverse transcriptase polymerase chain reaction for diagnosing influenza infections in respiratory samples from a children's hospital.

Journal of clinical microbiology ·Vol. 31 ·No. 8 ·1993-08-00 ·Pages 2218-21

Claas EC, van Milaan AJ, Sprenger MJ, Ruiten-Stuiver M, Arron GI, Rothbarth PH, Masurel N

Abstract

A prospective clinical evaluation of the reverse transcriptase polymerase chain reaction (RNA PCR) for detection of influenza viruses was carried out with specimens from 342 patients of a children's hospital in The Netherlands. The RNA PCR, carried out directly on the specimens without an organic extraction, showed a sensitivity and specificity which are superior to those of direct immunofluorescence and comparable to those of cell culture combined with immunofluorescence (culture/IF). Negative results can be obtained within 2 days by the RNA PCR but may take up to 14 days by culture/IF. Because culturing is the standard technique for the detection of respiratory viruses, at this moment there are no strong arguments to replace culture/IF with RNA PCR for the detection of influenza A virus.

MeSH Terms

Base Sequence Bronchoalveolar Lavage Fluid/microbiology Child Fluorescent Antibody Technique Humans Influenza, Human/diagnosis Molecular Sequence Data Nasopharynx/microbiology Orthomyxoviridae/genetics,isolation & purification Polymerase Chain Reaction Prospective Studies RNA, Viral/analysis Sensitivity and Specificity

Chemicals

RNA, Viral

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Claas E C

Department of Virology, Erasmus University, Rotterdam, The Netherlands.

van Milaan A J

Sprenger M J

Ruiten-Stuiver M

Arron G I

Rothbarth P H

Masurel N

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.31.8.2218-2221.1993

Published

1993-08-00

Pages

2218-21

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC265728

Subset

External Links

PubMed source DOI: 10.1128/jcm.31.8.2218-2221.1993 PMC Full Text View journal details

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Prospective application of reverse transcriptase polymerase chain reaction for diagnosing influenza infections in respiratory samples from a children's hospital.

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