A novel method employing UNG to avoid carry-over contamination in RNA-PCR.

Nucleic acids research ·Vol. 21 ·No. 16 ·1993-08-11 ·Pages 3917-8

Udaykumar, Epstein JS, Hewlett IK

Abstract

暂无摘要

MeSH Terms

Cell Line DNA Glycosylases HIV-1/genetics Humans N-Glycosyl Hydrolases/metabolism Polymerase Chain Reaction/methods RNA, Viral/isolation & purification Uracil-DNA Glycosidase

Chemicals

RNA, Viral DNA Glycosylases N-Glycosyl Hydrolases Uracil-DNA Glycosidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Udaykumar

Laboratory of Molecular Virology, FDA/CBER, Rockville, MD 20852-1448.

Epstein J S

Hewlett I K

References (3)

3 references, click to expand

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling.
Biotechniques. 1992 Aug;13(2):180-4 PMID: 1389144
Avoiding false positives with PCR.
Nature. 1989 May 18;339(6221):237-8 PMID: 2716852

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/21.16.3917

Published

1993-08-11

Pages

3917-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC309940

Subset

External Links

PubMed source DOI: 10.1093/nar/21.16.3917 PMC Full Text View journal details

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A novel method employing UNG to avoid carry-over contamination in RNA-PCR.

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