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PMID: 8366044 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme.

Journal of bacteriology ·Vol. 175 ·No. 17 ·1993-09-00 ·Pages 5604-10

Carter JR, Franden MA, Aebersold R, McHenry CS

Abstract

The gene encoding the psi subunit of DNA polymerase III holoenzyme, holD, was identified and isolated by an approach in which peptide sequence data were used to obtain a DNA hybridization probe. The gene, which maps to 99.3 centisomes, was sequenced and found to be identical to a previously uncharacterized open reading frame that overlaps the 5' end of rimI by 29 bases, contains 411 bp, and is predicted to encode a protein of 15,174 Da. When expressed in a plasmid that also expressed holC, holD directed expression of the psi subunit to about 3% of total soluble protein.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Polymerase III/biosynthesis,chemistry,genetics,isolation & purification DNA, Bacterial Escherichia coli/enzymology,genetics Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Molecular Sequence Data Open Reading Frames Plasmids Polymerase Chain Reaction
Chemicals
DNA, Bacterial DNA Polymerase III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carter J R
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262.
Franden M A
Aebersold R
McHenry C S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.17.5604-5610.1993
Published
1993-09-00
Pages
5604-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206617
Subset
IM
Grants
NIGMS NIH HHS · GM36255 · United States
Databases
GENBANK
UNKNOWN
External Links
PubMed source DOI: 10.1128/jb.175.17.5604-5610.1993 PMC Full Text View journal details
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