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PMID: 83650 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of bleomycin with DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 12 ·1978-12-00 ·Pages 5983-7

Takeshita M, Grollman AP, Ohtsubo E, Ohtsubo H

Abstract

The sequence of oligonucleotides produced by the action of bleomycin and ferrous ion on double- and single-stranded DNA has been determined. In the presence of ferrous ion, bleomycin promotes cleavage at G-T and G-C sequences, while high concentrations of ferrous ion alone result in strand scission that is not base specific. In the presence of bleomycin and ferrous ion, pyrimidine bases located to the 3' side of guanosine are released preferentially and a low molecular weight product that forms a chromophore with thiobarbituric acid is produced from the deoxyribose moiety. Oligonucleotides produced by the action of bleomycin differ slightly in electrophoretic mobility from those produced by chemical or enzymatic cleavage. A model is proposed to explain the interactions of bleomycin with DNA.

MeSH Terms
Base Sequence Binding Sites Bleomycin/metabolism Coliphages DNA, Bacterial/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism Deoxyribose/metabolism Escherichia coli Ferrous Compounds/pharmacology Hydrolysis Mercaptoethanol/pharmacology
Chemicals
DNA, Bacterial DNA, Single-Stranded DNA, Viral Ferrous Compounds Bleomycin Deoxyribose Mercaptoethanol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takeshita M
Grollman A P
Ohtsubo E
Ohtsubo H
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.12.5983
Published
1978-12-00
Pages
5983-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393101
Subset
IM
Databases
GENBANK
M10699, M10701
External Links
PubMed source DOI: 10.1073/pnas.75.12.5983 PMC Full Text View journal details
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