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PMID: 8358030 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

WIP1, a wound-inducible gene from maize with homology to Bowman-Birk proteinase inhibitors.

Plant molecular biology ·Vol. 22 ·No. 5 ·1993-08-00 ·Pages 783-92

Rohrmeier T, Lehle L

Abstract

We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 residues with a calculated molecular mass of 11 kDa and a typical N-terminal signal sequence. The protein has about 30% identity with various Bowman-Birk type proteinase inhibitors. Most interestingly, it is novel in that it is double-headed with exclusive specificity for chymotrypsin. WIP1 is strongly wound-induced in contrast to other members of the Bowman-Birk proteinase inhibitor family, which occur in seeds and are regulated during development. The response is fast, similar to defence-induced genes, and measurable as early as 30 min after wounding. Induction can also be evoked in the intact coleoptiles and the signal is systematically transmitted in the coleoptile to adjacent regions of the wounded area. Isolation and analysis of the corresponding genomic clone reveals that WIP1 contains an intron of 90 nucleotides.

Related Genes
WIP1
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Gene Expression Regulation Molecular Sequence Data Plant Proteins/chemistry,genetics Sequence Homology, Amino Acid Serine Proteinase Inhibitors Trypsin Inhibitor, Bowman-Birk Soybean/chemistry,genetics Zea mays/cytology,genetics,growth & development
Chemicals
Plant Proteins Serine Proteinase Inhibitors Trypsin Inhibitor, Bowman-Birk Soybean WIP1 protein, Zea mays DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rohrmeier T
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany.
Lehle L
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00027365
Published
1993-08-00
Pages
783-92
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
X71396
External Links
PubMed source DOI: 10.1007/BF00027365 View journal details
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