Home LiteratureArticle Details
PMID: 8350346 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The evolution of the thrombospondin gene family.

Journal of molecular evolution ·Vol. 36 ·No. 6 ·1993-06-00 ·Pages 509-16

Lawler J, Duquette M, Urry L, McHenry K, Smith TF

Abstract

Thrombospondin-1 is an adhesive glycoprotein that is involved in cellular attachment, spreading, migration, and proliferation. To date, four genes have been identified that encode for the members of the thrombospondin gene family. These four genes are homologous to each other in the EGF-like (type 2) repeats, the calcium-binding (type 3) motifs, and the COOH-terminal. The latter has been reported to be a cell-binding domain in thrombospondin-1. Phylogenetic trees have been constructed from the multisequence alignment of thrombospondin sequences from human, mouse, chicken, and frog. Two different algorithms generate comparable results in terms of the topology and the branch lengths. The analysis indicates that an early form of the thrombospondin gene duplicated about 925 million years ago. The gene duplication that produced the thrombospondin-1 and -2 branches of the family is predicted to have occurred 583 million years ago, whereas the gene duplication that produced the thrombospondin-3 and -4 branches of the family is predicted to have occurred 644 million years ago. These results indicate that the members of the thrombospondin gene family have existed throughout the evolution of the animal kingdom and thus probably participate in functions that are common to most of its members.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chickens/genetics Genes Humans Mice/genetics Molecular Sequence Data Multigene Family Phylogeny Platelet Membrane Glycoproteins/genetics Sequence Alignment Sequence Homology, Amino Acid Species Specificity Thrombospondins Xenopus laevis/genetics
Chemicals
Platelet Membrane Glycoproteins Thrombospondins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lawler J
Department of Pathology, Brigham and Women's Hospital, Boston, MA.
Duquette M
Urry L
McHenry K
Smith T F
References (49)
49 references, click to expand
  1. Cooperative binding of calcium to thrombospondin. The effect of calcium on the circular dichroism and limited tryptic digestion of thrombospondin.
    J Biol Chem. 1983 Oct 25;258(20):12098-101 PMID: 6630182
  2. The structural and functional properties of thrombospondin.
    Blood. 1986 May;67(5):1197-209 PMID: 3516249
  3. F-spondin: a gene expressed at high levels in the floor plate encodes a secreted protein that promotes neural cell adhesion and neurite extension.
    Cell. 1992 Apr 3;69(1):95-110 PMID: 1555244
  4. Heparan sulfate-mediated binding of epithelial cell surface proteoglycan to thrombospondin.
    J Biol Chem. 1989 Feb 15;264(5):2885-9 PMID: 2521631
  5. Cloning and sequencing of chicken thrombospondin.
    J Biol Chem. 1991 May 5;266(13):8039-43 PMID: 2022631
  6. A monoclonal antibody against human thrombospondin inhibits platelet aggregation.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3472-6 PMID: 2582413
  7. Molecular biology of the extracellular matrix proteins.
    Biol Rev Camb Philos Soc. 1988 Nov;63(4):465-507 PMID: 2461746
  8. The GPIIB-IIIa-like complex may function as a human melanoma cell adhesion receptor for thrombospondin.
    Exp Cell Res. 1989 Jun;182(2):473-81 PMID: 2470606
  9. A second thrombospondin gene in the mouse is similar in organization to thrombospondin 1 but does not respond to serum.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8636-40 PMID: 1924325
  10. The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins.
    J Cell Biol. 1986 Nov;103(5):1635-48 PMID: 2430973
  11. Thrombospondin modulates focal adhesions in endothelial cells.
    J Cell Biol. 1989 Sep;109(3):1309-19 PMID: 2768342
  12. Experimental phylogenetics: generation of a known phylogeny.
    Science. 1992 Jan 31;255(5044):589-92 PMID: 1736360
  13. Mapping of epitopes for monoclonal antibodies against human platelet thrombospondin with electron microscopy and high sensitivity amino acid sequencing.
    J Cell Biol. 1985 Oct;101(4):1434-41 PMID: 2413043
  14. Biological activities of peptides and peptide analogues derived from common sequences present in thrombospondin, properdin, and malarial proteins.
    J Cell Biol. 1992 Jan;116(1):209-17 PMID: 1730745
  15. Albumin phylogeny for clawed frogs (Xenopus).
    Science. 1977 Feb 25;195(4280):785-7 PMID: 65013
  16. The phylogenetic history of immunodeficiency viruses.
    Nature. 1988 Jun 9;333(6173):573-5 PMID: 3131682
  17. Xenopus laevis integrins. Structural conservation and evolutionary divergence of integrin beta subunits.
    J Biol Chem. 1988 Apr 15;263(11):5333-40 PMID: 2833505
  18. Automatic generation of primary sequence patterns from sets of related protein sequences.
    Proc Natl Acad Sci U S A. 1990 Jan;87(1):118-22 PMID: 2296575
  19. Progressive sequence alignment as a prerequisite to correct phylogenetic trees.
    J Mol Evol. 1987;25(4):351-60 PMID: 3118049
  20. Mapping of the thrombospondin gene to human chromosome 15 and mouse chromosome 2 by in situ hybridization.
    Genomics. 1990 May;7(1):123-6 PMID: 2335352
  21. The structure of human platelet thrombospondin.
    J Biol Chem. 1985 Mar 25;260(6):3762-72 PMID: 2579080
  22. Cell-adhesive motif in region II of malarial circumsporozoite protein.
    Science. 1990 Sep 28;249(4976):1574-7 PMID: 2120774
  23. Structure and chromosomal localization of the human thrombospondin gene.
    Genomics. 1990 Apr;6(4):685-91 PMID: 2341158
  24. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  25. EGF-like domains in extracellular matrix proteins: localized signals for growth and differentiation?
    FEBS Lett. 1989 Jul 17;251(1-2):1-7 PMID: 2666164
  26. Thrombospondin 3 (Thbs3), a new member of the thrombospondin gene family.
    J Biol Chem. 1992 Jun 15;267(17):12192-6 PMID: 1601886
  27. Human carcinoma cells express receptors for distinct domains of thrombospondin.
    Cancer Res. 1991 Mar 15;51(6):1645-50 PMID: 1705477
  28. A second, expressed thrombospondin gene (Thbs2) exists in the mouse genome.
    J Biol Chem. 1991 Jul 15;266(20):12821-4 PMID: 1712771
  29. Platelet thrombospondin modulates endothelial cell adhesion, motility, and growth: a potential angiogenesis regulatory factor.
    J Cell Biol. 1990 Aug;111(2):765-72 PMID: 1696271
  30. Expression and mutagenesis of thrombospondin.
    Biochemistry. 1992 Feb 4;31(4):1173-80 PMID: 1734965
  31. Detecting distant homologies of mosaic proteins. Analysis of the sequences of thrombomodulin, thrombospondin complement components C9, C8 alpha and C8 beta, vitronectin and plasma cell membrane glycoprotein PC-1.
    J Mol Biol. 1988 Aug 20;202(4):689-96 PMID: 2459396
  32. The properdin-like type I repeats of human thrombospondin contain a cell attachment site.
    J Cell Biol. 1991 Mar;112(5):1031-40 PMID: 1999454
  33. A highly conserved amino-acid sequence in thrombospondin, properdin and in proteins from sporozoites and blood stages of a human malaria parasite.
    Nature. 1988 Sep 1;335(6185):79-82 PMID: 3045563
  34. Thrombospondin II: partial cDNA sequence, chromosome location, and expression of a second member of the thrombospondin gene family in humans.
    Genomics. 1992 Mar;12(3):421-9 PMID: 1559694
  35. Characterization of the mouse thrombospondin gene and evaluation of the role of the first intron in human gene expression.
    J Biol Chem. 1990 Sep 25;265(27):16691-8 PMID: 2398070
  36. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.
    J Mol Evol. 1980 Dec;16(2):111-20 PMID: 7463489
  37. Chinese hamster ovary cell adhesion to human platelet thrombospondin is dependent on cell surface heparan sulfate proteoglycan.
    J Clin Invest. 1989 Mar;83(3):994-1001 PMID: 2522106
  38. Pattern-induced multi-sequence alignment (PIMA) algorithm employing secondary structure-dependent gap penalties for use in comparative protein modelling.
    Protein Eng. 1992 Jan;5(1):35-41 PMID: 1631044
  39. Characterization of mouse thrombospondin 2 sequence and expression during cell growth and development.
    J Biol Chem. 1992 Feb 15;267(5):3274-81 PMID: 1371115
  40. Properdin, the terminal complement components, thrombospondin and the circumsporozoite protein of malaria parasites contain similar sequence motifs.
    Nature. 1988 Sep 1;335(6185):82-5 PMID: 3045564
  41. Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors.
    J Cell Biol. 1988 Dec;107(6 Pt 1):2351-61 PMID: 2848850
  42. Cellular attachment to thrombospondin. Cooperative interactions between receptor systems.
    J Biol Chem. 1991 Jan 25;266(3):1740-5 PMID: 1703153
  43. Biochemical evolution.
    Annu Rev Biochem. 1977;46:573-639 PMID: 409339
  44. The Drosophila melanogaster stranded at second (sas) gene encodes a putative epidermal cell surface receptor required for larval development.
    Dev Biol. 1992 Jun;151(2):431-45 PMID: 1339334
  45. Partial amino acid sequence of human thrombospondin as determined by analysis of cDNA clones: homology to malarial circumsporozoite proteins.
    Biochemistry. 1986 Dec 30;25(26):8418-25 PMID: 3030396
  46. Antibodies against a 23Kd heparin binding fragment of thrombospondin inhibit platelet aggregation.
    Biochem Biophys Res Commun. 1984 Oct 15;124(1):290-5 PMID: 6497883
  47. Characterization of a cDNA encoding the heparin and collagen binding domains of human thrombospondin.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5449-53 PMID: 3461443
  48. Why genes in pieces?
    Nature. 1978 Feb 9;271(5645):501 PMID: 622185
  49. Characterization of the murine thrombospondin gene.
    Genomics. 1991 Nov;11(3):587-600 PMID: 1774063
Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF00556355
Published
1993-06-00
Pages
509-16
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Grants
NHLBI NIH HHS · HL28749 · United States
NHLBI NIH HHS · HL42443 · United States
NCRR NIH HHS · RR02275 · United States
External Links
PubMed source DOI: 10.1007/BF00556355 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2