Sensitivity of culture compared with that of polymerase chain reaction for detection of Helicobacter pylori from antral biopsy samples.

Journal of clinical microbiology ·Vol. 31 ·No. 7 ·1993-07-00 ·Pages 1918-20

van Zwet AA, Thijs JC, Kooistra-Smid AM, Schirm J, Snijder JA

Abstract

Polymerase chain reaction (PCR) was used to test the sensitivity of standard bacteriological culture of Helicobacter pylori. The tests were concordant in 97% of cases. PCR did not detect more treatment failures after triple treatment than did the standard culture method. In the present study, culture was as sensitive as PCR for the detection of H. pylori.

MeSH Terms

Bacteriological Techniques/statistics & numerical data Base Sequence DNA, Bacterial/genetics Evaluation Studies as Topic Gastritis/diagnosis,microbiology Gastroscopy Helicobacter Infections/diagnosis,microbiology Helicobacter pylori/genetics,isolation & purification Humans Molecular Sequence Data Polymerase Chain Reaction/statistics & numerical data Pyloric Antrum/microbiology Sensitivity and Specificity

Chemicals

DNA, Bacterial

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

van Zwet A A

Regional Public Health Laboratory, Groningen, The Netherlands.

Thijs J C

Kooistra-Smid A M

Schirm J

Snijder J A

References (10)

10 references, click to expand

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Effect of treatment of Helicobacter pylori infection on the long-term recurrence of gastric or duodenal ulcer. A randomized, controlled study.
Ann Intern Med. 1992 May 1;116(9):705-8 PMID: 1558340
Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.
J Clin Microbiol. 1991 Nov;29(11):2543-9 PMID: 1723072
Sensitive detection of Helicobacter pylori by using polymerase chain reaction.
J Clin Microbiol. 1992 Jan;30(1):192-200 PMID: 1734052
Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction.
J Clin Microbiol. 1992 Jan;30(1):54-8 PMID: 1370850
Diagnostic value of decreasing IgG, IgA, and IgM antibody titres after eradication of Helicobacter pylori.
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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.31.7.1918-1920.1993

Published

1993-07-00

Pages

1918-20

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC265660

Subset

External Links

PubMed source DOI: 10.1128/jcm.31.7.1918-1920.1993 PMC Full Text View journal details

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Sensitivity of culture compared with that of polymerase chain reaction for detection of Helicobacter pylori from antral biopsy samples.

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