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PMID: 8346029 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human snRNP polypeptide D1 promotes pre-mRNA splicing in yeast and defines nonessential yeast Smd1p sequences.

Nucleic acids research ·Vol. 21 ·No. 15 ·1993-07-25 ·Pages 3501-5

Rymond BC, Rokeach LA, Hoch SO

Abstract

Parallel investigations of yeast and metazoan pre-mRNA splicing have documented enormous complexity in the nucleic acid and protein components of the cellular splicing apparatus, the spliceosome. The degree to which yeast and metazoan spliceosomal proteins differ in composition and structure is currently unknown. In this report we demonstrate that the human small nuclear ribonucleoprotein (snRNP) polypeptide D1 complements the cell lethality, splicing deficiency, and snRNA instability phenotypes associated with a yeast smd1 null allele. Mutational analysis of yeast SMD1, guided by a comparison of the predicted yeast and human proteins, reveals that a large, nonconserved portion of Smd1p is dispensable for biological activity. These observations firmly establish D1 as an essential component of the cellular splicing apparatus and suggest that yeast and metazoa are remarkably similar in the polypeptides guiding early snRNP assembly.

Related Genes
SMD1
MeSH Terms
Alleles Amino Acid Sequence Autoantigens Base Sequence Fungal Proteins/chemistry,genetics Genes, Fungal Humans Molecular Sequence Data Mutagenesis RNA Precursors/genetics RNA Splicing/drug effects RNA, Small Nuclear/metabolism Ribonucleoproteins, Small Nuclear/chemistry,genetics,pharmacology Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spliceosomes/metabolism snRNP Core Proteins
Chemicals
Autoantigens Fungal Proteins RNA Precursors RNA, Small Nuclear Ribonucleoproteins, Small Nuclear SMD1 protein, S cerevisiae SNRPD1 protein, human Saccharomyces cerevisiae Proteins snRNP Core Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rymond B C
T.H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-0225.
Rokeach L A
Hoch S O
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.15.3501
Published
1993-07-25
Pages
3501-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331451
Subset
IM
Grants
NIGMS NIH HHS · GM42476 · United States
External Links
PubMed source DOI: 10.1093/nar/21.15.3501 PMC Full Text View journal details
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