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PMID: 8343130 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Non-catalytic activation of phospholipase C-gamma 1 in vitro by epidermal growth factor receptor.

The Biochemical journal ·Vol. 293 ( Pt 2) ·1993-07-15 ·Pages 507-11

Hernández-Sotomayor SM, Carpenter G

Abstract

To investigate the possible functional role of epidermal growth factor (EGF) receptor-phospholipase C-gamma 1 (PLC-gamma 1) complexes, we have measured PLC-gamma 1 activity in vitro in the absence or presence of purified EGF receptor. Immunoprecipitates of PLC-gamma 1 from control A-431 cells were incubated without or with purified EGF receptor in the absence or presence of ATP. Under these conditions the EGF receptor increased non-tyrosine-phosphorylated PLC-gamma 1 activity 3-4-fold in the absence or presence of ATP, but increased tyrosine-phosphorylated and activated PLC-gamma 1 by only 20-50%. Both basal and autophosphorylated forms of the purified EGF receptor increased the activity of the non-tyrosine-phosphorylated PLC-gamma 1, and stoichiometric levels of purified receptor were required to increase PLC activity. Other tyrosine kinases such as the platelet-derived growth factor receptor and erbB-2, but not the insulin receptor, also stimulated PLC-gamma 1 activity. PLC-gamma 1 activity could be activated with the kinase-negative EGF receptor, but a C-terminal truncated receptor was much less effective. Purified EGF receptor could also activate PLC-beta 1, but with a much decreased potency compared with PLC-gamma 1. Our results suggest that in vitro the EGF receptor can increase PLC-gamma 1 activity independently of tyrosine phosphorylation.

MeSH Terms
3T3 Cells Animals Catalysis Cattle Cells, Cultured Enzyme Activation ErbB Receptors/metabolism Isoenzymes/metabolism Mice Phosphorylation Substrate Specificity Type C Phospholipases/metabolism Tyrosine/metabolism
Chemicals
Isoenzymes Tyrosine ErbB Receptors Type C Phospholipases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hernández-Sotomayor S M
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.
Carpenter G
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32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2930507
Published
1993-07-15
Pages
507-11
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1134390
Subset
IM
Grants
NCI NIH HHS · CA43720 · United States
FIC NIH HHS · TW04600 · United States
External Links
PubMed source DOI: 10.1042/bj2930507 PMC Full Text View journal details
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