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PMID: 8341669 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of a short interspersed repetitive element from tobacco that exhibits sequence homology to specific tRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 14 ·1993-07-15 ·Pages 6562-6

Yoshioka Y, Matsumoto S, Kojima S, Ohshima K, Okada N, Machida Y

Abstract

We have characterized a family of tRNA-derived short interspersed repetitive elements (SINEs) in the tobacco genome. Members of this family of SINEs, designated TS, have a composite structure and include a region structurally similar to a rabbit tRNA(Lys), a tRNA-unrelated region, and a TTG repeat of variable length at the 3' end. Southern blot hybridization, together with a search of the GenBank data base, showed that various plants belonging to the families Solanaceae and Convolvulaceae contain sequences homologous to the TS family in the introns and flanking regions of many genes, whereas Arabidopsis in the family Cruciferae and several species of monocoytledonous plants do not. The TS family is widely involved in structural and genetic variations in the genomes of many plants that belong to the order Tubiflorae. All of nine sequences identified in a data base search are truncated at their 5' regions and lack the tRNA-related region of the TS family. We characterized the entire sequence of the members of the TS family and found that this family can be categorized as a member of a group of SINEs with a tRNA(Lys)-like structure, as can several animal SINEs. The TS family can be divided into two major subfamilies by analysis of diagnostic positions, and one of the subfamilies is clearly younger than the other. Amplification of many copies of the full sequence of the younger subfamily occurred during the recent evolution of the tobacco lineage. We also discuss mechanisms that could be involved in the generation of SINEs in animals and also in plants.

MeSH Terms
Base Sequence Biological Evolution Gene Amplification/genetics Gene Library Genes, Plant/genetics Genome Introns/genetics Molecular Sequence Data Plants/genetics Plants, Toxic RNA, Transfer/genetics RNA, Transfer, Lys/genetics Regulatory Sequences, Nucleic Acid/genetics Repetitive Sequences, Nucleic Acid/genetics Sequence Homology, Nucleic Acid Tobacco/genetics Transcription, Genetic
Chemicals
RNA, Transfer, Lys RNA, Transfer
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yoshioka Y
Department of Biology, School of Science, Nagoya University, Japan.
Matsumoto S
Kojima S
Ohshima K
Okada N
Machida Y
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.14.6562
Published
1993-07-15
Pages
6562-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46972
Subset
IM
Databases
GENBANK
M29041, X60506, X67599
External Links
PubMed source DOI: 10.1073/pnas.90.14.6562 PMC Full Text View journal details
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