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PMID: 8341260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Saccharopolyspora hirsuta 367 encodes clustered genes similar to ketoacyl synthase, ketoacyl reductase, acyl carrier protein, and biotin carboxyl carrier protein.

Molecular & general genetics : MGG ·Vol. 240 ·No. 1 ·1993-07-00 ·Pages 146-50

Le Gouill C, Desmarais D, Déry CV

Abstract

The actI gene, encoding a component of the actinorhodin polyketide synthase of Streptomyces coelicolor, was used to identify and clone a homologous 11.7 kb BamHI DNA fragment from Saccharopolyspora hirsuta 367. The cloned fragment complemented actinorhodin production in a strain of Streptomyces coelicolor bearing a mutant actI gene. The DNA sequence of a 5.1 kb fragment revealed 6 open reading frames (ORF). ORF1 does not resemble any known DNA or deduced protein sequence, while the deduced protein sequence of ORF2 resembles that of biotin carboxyl carrier proteins. Based on the similarity to deduced protein sequences from cloned genes of polyketide producers, ORF3 would code for a ketoreductase, ORF4 and ORF5 for the putative heterodimeric beta-ketoacyl synthase, and ORF6 for an acyl carrier protein.

Related Genes

actI

MeSH Terms

3-Oxoacyl-(Acyl-Carrier-Protein) Reductase 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics Alcohol Oxidoreductases/genetics Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Carrier Proteins/genetics Cloning, Molecular Genes, Bacterial/genetics Molecular Sequence Data Multigene Family/genetics Open Reading Frames/genetics Saccharopolyspora/enzymology,genetics Sequence Homology

Chemicals

Bacterial Proteins Carrier Proteins Alcohol Oxidoreductases 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Le Gouill C

Département de Biologie, Faculté des Sciences, Université de Sherbrooke (Québec), Canada.

Desmarais D

Déry C V

References (20)

20 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00276894

Published

1993-07-00

Pages

146-50

Language

English

Region

Germany

NLM ID

0125036

Subset

Databases

GENBANK

M98258

External Links

PubMed source DOI: 10.1007/BF00276894

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Saccharopolyspora hirsuta 367 encodes clustered genes similar to ketoacyl synthase, ketoacyl reductase, acyl carrier protein, and biotin carboxyl carrier protein.

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