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PMID: 8334162 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The role of the beta-subunit of initiation factor eIF-2 in initiation complex formation.

Biochimica et biophysica acta ·Vol. 1174 ·No. 1 ·1993-07-18 ·Pages 117-21

Flynn A, Oldfield S, Proud CG

Abstract

The functional properties of preparations of protein synthesis initiation factor eIF-2 which lack the beta-subunit (as confirmed immunologically) were compared with those of the heterotrimeric factor. The former can bind guanine nucleotides but not initiator tRNA, and also exhibits a substantially reduced rate of initiation factor eIF-2B-mediated GDP/GTP-exchange.

MeSH Terms

Animals Eukaryotic Initiation Factor-2/chemistry,metabolism Guanine Nucleotide Exchange Factors Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Proteins/metabolism RNA, Transfer, Met/metabolism Rabbits Reticulocytes/metabolism

Chemicals

Eukaryotic Initiation Factor-2 Guanine Nucleotide Exchange Factors Proteins RNA, Transfer, Met Guanosine Diphosphate Guanosine Triphosphate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Flynn A

Department of Biochemistry, School of Medical Sciences, University of Bristol, UK.

Oldfield S

Proud C G

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0167-4781(93)90105-m

Published

1993-07-18

Pages

117-21

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0167-4781(93)90105-m

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The role of the beta-subunit of initiation factor eIF-2 in initiation complex formation.

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