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PMID: 8327462 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Peroxidase-dependent metal-independent oxidation of low density lipoprotein in vitro: a model for in vivo oxidation?

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 13 ·1993-07-01 ·Pages 5929-33

Wieland E, Parthasarathy S, Steinberg D

Abstract

Oxidative modification of low density lipoprotein is believed to be an important pathway by which the lipoprotein becomes atherogenic. The in vitro systems for oxidative modification of low density lipoprotein thus far described all appear to depend upon the presence in the medium of free transition metal ions (copper or iron). In vivo, on the other hand, these metals are present almost exclusively in tightly complexed forms that do not catalyze oxidative modification. The present studies describe oxidation of low density lipoprotein in a simple system that does not depend upon the presence of added free metal ions. It requires the presence of horseradish peroxidase and either hydrogen peroxide or lipid hydroperoxides.

MeSH Terms
Free Radicals Horseradish Peroxidase/pharmacology Humans Hydrogen Peroxide/pharmacology Linoleic Acid Linoleic Acids/metabolism Lipoproteins, LDL/metabolism Lipoxygenase/pharmacology Metals/pharmacology Oxidation-Reduction Peroxidases/metabolism
Chemicals
Free Radicals Linoleic Acids Lipoproteins, LDL Metals Linoleic Acid Hydrogen Peroxide Horseradish Peroxidase Peroxidases Lipoxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wieland E
Department of Medicine, University of California, San Diego, La Jolla 92093-0682.
Parthasarathy S
Steinberg D
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.13.5929
Published
1993-07-01
Pages
5929-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46840
Subset
IM
Grants
NHLBI NIH HHS · HL-14197 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.13.5929 PMC Full Text View journal details
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