Home LiteratureArticle Details
PMID: 8318890 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and expression of chicken skeletal muscle troponin I in Escherichia coli: the role of rare codons on the expression level.

Protein science : a publication of the Protein Society ·Vol. 2 ·No. 6 ·1993-06-00 ·Pages 1053-6

Quaggio RB, Ferro JA, Monteiro PB, Reinach FC

Abstract

暂无摘要

MeSH Terms
Animals Base Sequence Calcium/metabolism Chickens Cloning, Molecular Codon/genetics DNA/genetics Escherichia coli/genetics Gene Expression Molecular Sequence Data Muscles/metabolism Myosins/antagonists & inhibitors Recombinant Proteins/genetics,metabolism Troponin/genetics,metabolism Troponin C Troponin I
Chemicals
Codon Recombinant Proteins Troponin Troponin C Troponin I DNA Myosins Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Quaggio R B
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
Ferro J A
Monteiro P B
Reinach F C
References (25)
25 references, click to expand
  1. The relaxing protein system of striated muscle. Resolution of the troponin complex into inhibitory and calcium ion-sensitizing factors and their relationship to tropomyosin.
    Biochem J. 1969 Dec;115(5):993-1004 PMID: 4243353
  2. Isolation, expression, and mutation of a rabbit skeletal muscle cDNA clone for troponin I. The role of the NH2 terminus of fast skeletal muscle troponin I in its biological activity.
    J Biol Chem. 1992 Dec 15;267(35):25407-13 PMID: 1339446
  3. The interaction of the calcium-binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I).
    Biochem J. 1974 Feb;137(2):145-54 PMID: 4824205
  4. The calcium and magnesium binding sites on troponin and their role in the regulation of myofibrillar adenosine triphosphatase.
    J Biol Chem. 1975 Jun 25;250(12):4628-33 PMID: 124731
  5. Comparison of amino acid sequence of troponin I from different striated muscles.
    Nature. 1978 Jan 5;271(5640):31-5 PMID: 146828
  6. Molecular structure of troponin C from chicken skeletal muscle at 3-angstrom resolution.
    Science. 1985 Feb 22;227(4689):945-8 PMID: 3969570
  7. Structure of the calcium regulatory muscle protein troponin-C at 2.8 A resolution.
    Nature. 1985 Feb 21-27;313(6004):653-9 PMID: 3974698
  8. The chicken fast skeletal troponin I gene: exon organization and sequence.
    Nucleic Acids Res. 1986 Apr 25;14(8):3377-90 PMID: 3010234
  9. Structural aspects of troponin-tropomyosin regulation of skeletal muscle contraction.
    Annu Rev Biophys Biophys Chem. 1987;16:535-59 PMID: 2954560
  10. Cloning, expression, and site-directed mutagenesis of chicken skeletal muscle troponin C.
    J Biol Chem. 1988 Feb 15;263(5):2371-6 PMID: 2963002
  11. Cloning, sequencing and expression of a full-length rabbit fast skeletal troponin-C cDNA.
    FEBS Lett. 1988 Feb 8;228(1):22-6 PMID: 3277860
  12. The AGG codon is translated slowly in E. coli even at very low expression levels.
    Nucleic Acids Res. 1988 Apr 11;16(7):3013-24 PMID: 3285325
  13. Synthesis of a troponin C cDNA and expression of wild-type and mutant proteins in Escherichia coli.
    J Biol Chem. 1988 Sep 25;263(27):13962-9 PMID: 2971047
  14. Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes.
    Nucleic Acids Res. 1990 Mar 25;18(6):1465-73 PMID: 2109307
  15. Control of prokaryotic translational initiation by mRNA secondary structure.
    Prog Nucleic Acid Res Mol Biol. 1990;38:1-35 PMID: 2183291
  16. Inhibition of mutant troponin C activity by an intra-domain disulphide bond.
    Nature. 1990 May 10;345(6271):132-5 PMID: 2110625
  17. Probing the calcium-induced conformational transition of troponin C with site-directed mutants.
    Nature. 1990 May 10;345(6271):182-4 PMID: 2186281
  18. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  19. Effects of mutations in the central helix of troponin C on its biological activity.
    J Biol Chem. 1991 Mar 25;266(9):5711-5 PMID: 2005108
  20. Calcium binding induces conformational changes in muscle regulatory proteins.
    Trends Biochem Sci. 1991 Feb;16(2):53-7 PMID: 1858133
  21. Function of the N-terminal calcium-binding sites in cardiac/slow troponin C assessed in fast skeletal muscle fibers.
    J Biol Chem. 1991 Aug 15;266(23):14881-4 PMID: 1869527
  22. Mutation of the high affinity calcium binding sites in cardiac troponin C.
    J Biol Chem. 1992 Jan 15;267(2):825-31 PMID: 1530938
  23. Construction and characterization of a spectral probe mutant of troponin C: application to analyses of mutants with increased Ca2+ affinity.
    Biochemistry. 1992 Jul 21;31(28):6545-53 PMID: 1633166
  24. Molecular mechanism of troponin-C function.
    J Muscle Res Cell Motil. 1992 Aug;13(4):383-93 PMID: 1401036
  25. Reconstitution of troponin activity from three protein components.
    J Biol Chem. 1971 Jul 10;246(13):4226-33 PMID: 4253596
Article Info
Journal
Protein science : a publication of the Protein Society
Abbr.
Protein Sci
ISSN
0961-8368
Published
1993-06-00
Pages
1053-6
Language
English
Region
United States
NLM ID
9211750
PMCID
PMC2142394
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com