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PMID: 8307323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements.

Genetics ·Vol. 135 ·No. 4 ·1993-12-00 ·Pages 1063-76

Smith D, Wohlgemuth J, Calvi BR, Franklin I, Gelbart WM

Abstract

P element enhancer trapping has become an indispensable tool in the analysis of the Drosophila melanogaster genome. However, there is great variation in the mutability of loci by these elements such that some loci are relatively refractory to insertion. We have developed the hobo transposable element for use in enhancer trapping and we describe the results of a hobo enhancer trap screen. In addition, we present evidence that a hobo enhancer trap element has a pattern of insertion into the genome that is different from the distribution of P elements in the available database. Hence, hobo insertion may facilitate access to genes resistant to P element insertion.

MeSH Terms
Animals Animals, Genetically Modified Chromosomes DNA Transposable Elements Drosophila melanogaster/embryology,genetics Enhancer Elements, Genetic Female Genes, Lethal Homozygote Male Mutagenesis
Chemicals
DNA Transposable Elements
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Smith D
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
Wohlgemuth J
Calvi B R
Franklin I
Gelbart W M
References (27)
27 references, click to expand
  1. Preferential transposition of Drosophila P elements to nearby chromosomal sites.
    Genetics. 1993 Feb;133(2):347-59 PMID: 8382177
  2. Insertional mutagenesis of the maize P gene by intragenic transposition of Ac.
    Genetics. 1992 May;131(1):199-209 PMID: 1317315
  3. Genetic instability in Drosophila melanogaster mediated by hobo transposable elements.
    Genetics. 1993 Feb;133(2):315-34 PMID: 8382175
  4. Controlling P element insertional mutagenesis.
    Trends Genet. 1988 Sep;4(9):254-8 PMID: 2853469
  5. The ins and outs of transposition. Molecular Mechanisms of Transposition and its Control, sponsored by the European Molecular Biology Organization, Roscoff, France, June 24-28, 1990.
    New Biol. 1990 Sep;2(9):787-92 PMID: 1980622
  6. Efficient and dispersed local P element transposition from Drosophila females.
    Genetics. 1993 Feb;133(2):361-73 PMID: 8382178
  7. The control of cell fate along the dorsal-ventral axis of the Drosophila embryo.
    Development. 1991 Sep;113(1):35-54 PMID: 1765005
  8. Mobilization of hobo elements residing within the decapentaplegic gene complex: suggestion of a new hybrid dysgenesis system in Drosophila melanogaster.
    Cell. 1987 May 22;49(4):497-505 PMID: 3032458
  9. Studies on the rate and site-specificity of P element transposition.
    Genetics. 1991 Mar;127(3):515-24 PMID: 1849859
  10. Transpositions of Modulator, a Component of the Variegated Pericarp Allele in Maize.
    Genetics. 1959 Jul;44(4):725-38 PMID: 17247852
  11. Toward cloning and mapping the genome of Drosophila.
    Science. 1991 Oct 11;254(5029):221-5 PMID: 1925579
  12. P-element-mediated enhancer detection: a versatile method to study development in Drosophila.
    Genes Dev. 1989 Sep;3(9):1288-300 PMID: 2558050
  13. Molecular mechanisms regulating Drosophila P element transposition.
    Annu Rev Genet. 1990;24:543-78 PMID: 1965103
  14. P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila.
    Genes Dev. 1989 Sep;3(9):1301-13 PMID: 2558051
  15. Detection in situ of genomic regulatory elements in Drosophila.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9123-7 PMID: 2827169
  16. Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.
    Genes Dev. 1989 Sep;3(9):1273-87 PMID: 2558049
  17. Drosophila laminin: sequence of B2 subunit and expression of all three subunits during embryogenesis.
    J Cell Biol. 1989 Nov;109(5):2441-53 PMID: 2808533
  18. An extensive 3' cis-regulatory region directs the imaginal disk expression of decapentaplegic, a member of the TGF-beta family in Drosophila.
    Development. 1991 Mar;111(3):657-66 PMID: 1908769
  19. Insertional mutagenesis of the Drosophila genome with single P elements.
    Science. 1988 Mar 4;239(4844):1121-8 PMID: 2830671
  20. The structure of hobo transposable elements and their insertion sites.
    EMBO J. 1986 Dec 20;5(13):3615-23 PMID: 16453744
  21. Generating lineage-specific markers to study Drosophila development.
    Dev Genet. 1991;12(3):238-52 PMID: 1651183
  22. Regulation and function of the Drosophila segmentation gene fushi tarazu.
    Cell. 1987 Sep 11;50(6):963-74 PMID: 2887293
  23. Drosophila substrate adhesion molecule: sequence of laminin B1 chain reveals domains of homology with mouse.
    Cell. 1988 May 6;53(3):463-73 PMID: 3365769
  24. Multiple upstream regulatory elements control the expression of the Drosophila white gene.
    EMBO J. 1985 Dec 16;4(13A):3501-8 PMID: 3004963
  25. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9 PMID: 3003748
  26. Reconstitutional mutagenesis of the maize P gene by short-range Ac transpositions.
    Genetics. 1992 Aug;131(4):939-56 PMID: 1325389
  27. Decapentaplegic transcripts are localized along the dorsal-ventral axis of the Drosophila embryo.
    EMBO J. 1987 Sep;6(9):2785-91 PMID: 3119329
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/135.4.1063
Published
1993-12-00
Pages
1063-76
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205738
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/135.4.1063 PMC Full Text View journal details
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