The PII protein in the cyanobacterium Synechococcus sp. strain PCC 7942 is modified by serine phosphorylation and signals the cellular N-status.
The glnB gene product (PII protein) from Synechococcus sp. has previously been identified among 32P-labeled proteins, and its modification state has been observed to depend on both the nitrogen source and the spectral light quality (N. F. Tsinoremas, A. M. Castets, M. A. Harrison, J. F. Allen, and N. Tandeau de Marsac, Proc. Natl. Acad. Sci. USA 88:4565-4569, 1991). As shown in this study, modification of the PII protein primarily responds to the N-status of the cell, and its light-dependent variations are are mediated through nitrate metabolism. Modification of the PII protein results in the appearance of three isomeric forms with increasing negative charge. Unlike its homolog counterparts characterized so far, PII in Synechococcus sp. is modified by phosphorylation on a serine residue, which represents a unique kind of protein modification in bacterial nitrogen signalling pathways.
17 references, click to expand
-
Modification of a glnB-like gene product by photosynthetic electron transport in the cyanobacterium Synechococcus 6301.FEBS Lett. 1990 May 7;264(1):25-8 PMID: 2110911
-
Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis.Methods Enzymol. 1991;201:10-21 PMID: 1943759
-
Dependence of nitrate utilization upon active CO2 fixation in Anacystis nidulans: a regulatory aspect of the interaction between photosynthetic carbon and nitrogen metabolism.Arch Biochem Biophys. 1985 Mar;237(2):396-401 PMID: 3919645
-
Regulation of glutamine synthetase adenylylation and deadenylylation by the enzymatic uridylylation and deuridylylation of the PII regulatory protein.Arch Biochem Biophys. 1973 Oct;158(2):514-25 PMID: 4150122
-
Cascade control of Escherichia coli glutamine synthetase. Properties of the PII regulatory protein and the uridylyltransferase-uridylyl-removing enzyme.J Biol Chem. 1975 Aug 25;250(16):6264-72 PMID: 239942
-
Identification of a putative eukaryotic-like protein kinase family in the developmental bacterium Myxococcus xanthus.J Bacteriol. 1992 Aug;174(16):5450-3 PMID: 1644772
-
Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.Proc Natl Acad Sci U S A. 1986 Aug;83(16):5909-13 PMID: 2874557
-
Photosynthetic electron transport controls nitrogen assimilation in cyanobacteria by means of posttranslational modification of the glnB gene product.Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4565-9 PMID: 1905010
-
Metabolite-sensitive, ATP-dependent, protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system in gram-positive bacteria.Biochimie. 1989 Sep-Oct;71(9-10):989-96 PMID: 2557095
-
Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PII protein and nucleotide sequence of its structural gene.J Biol Chem. 1987 Jun 25;262(18):8690-5 PMID: 2885322
-
Isolation and purification of cyanobacteria.Methods Enzymol. 1988;167:3-27 PMID: 3148836
-
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
-
Molecular characterization of ferredoxin-NADP+ oxidoreductase in cyanobacteria: cloning and sequence of the petH gene of Synechococcus sp. PCC 7002 and studies on the gene product.Biochemistry. 1992 Mar 31;31(12):3092-102 PMID: 1554697
-
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
-
Distinctive Light and CO(2)-Fixation Requirements of Nitrate and Ammonium Utilization by the Cyanobacterium Anacystis nidulans.Plant Physiol. 1986 Jun;81(2):686-8 PMID: 16664880
-
Identification of the Escherichia coli cya gene product as authentic adenylate cyclase.J Mol Biol. 1984 May 25;175(3):403-8 PMID: 6427472
-
A host-vector system for gene cloning in the cyanobacterium Anacystis nidulans R2.Plasmid. 1983 Sep;10(2):156-63 PMID: 6314409
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