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题目:: Radiosensitivity of CD45RO+ memory and CD45RO- naive T cells in culture.
作者:: Uzawa(A),Suzuki(G),Nakata(Y),Akashi(M),Ohyama(H),Akanuma(A)
状态:: 发布时间1994-01-21 , 更新时间 2013-11-21
期刊:: Radiat Res
摘要:: Radiosensitivities of various human T-cell subsets were investigated by a proliferation assay and by a single-cell gel electrophoresis assay. Each T-cell subset was purified using a cell sorter and was induced to proliferate by ionomycin and interleukin 2. Unsorted T cells showed biphasic dose-survival curves, indicating the heterogeneity of T cells in terms of radiosensitivity. Purified CD4+ helper and CD8+ killer T cells showed similar biphasic dose-survival curves. Hence both T-cell subsets were composed of cells of different radiosensitivity. The T-cell subsets belonging to different activation stages such as CD45RO+ memory and CD45RO- naive T cells had different dose-survival curves. The former was more radiosensitive than the latter. The high radiosensitivity of CD45RO+ cells was also demonstrated by single-cell gel electrophoresis after irradiation. This is the first demonstration that a particular cell surface marker on T cells is correlated with greater radiosensitivity.
语言:: eng
DOI:

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