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PMID: 8258343 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human IgM+IgD+ B cells, the major B cell subset in the peripheral blood, express V kappa genes with no or little somatic mutation throughout life.

European journal of immunology ·Vol. 23 ·No. 12 ·1993-12-00 ·Pages 3272-7

Klein U, Küppers R, Rajewsky K

Abstract

Peripheral blood B cells of a 67-year-old person were separated into IgM+IgD+, IgM+IgD-, and IgM-IgD- subsets, and nucleotide sequences of expressed immunoglobulin light chain variable (V) regions encoded by V kappa 3 and V kappa 4 gene family members were determined from amplified cDNA. V region sequences from IgM+IgD+ cells (the major B cell population in the blood) showed no or little somatic mutation (0.3%), in contrast to V kappa sequences from IgM+IgD- and IgM-IgD- B cells (2.0% and 3.9%, respectively). This suggests that in the human like in the mouse, and independently of age, somatically mutated memory B cells accumulate in the compartment of IgM-IgD- cells, whereas the IgM+IgD+ subpopulation consists of cells whose antibody repertoire is mainly determined by V region gene rearrangements and N-region insertion, at the molecular level. The somatically mutated IgM+IgD- cells may represent early descendants of IgM+IgD+ cells recruited into the memory cell compartment.

MeSH Terms

Aged Amino Acid Sequence B-Lymphocyte Subsets/immunology Base Sequence Gene Expression Genes, Immunoglobulin Humans Immunoglobulin D/analysis Immunoglobulin Joining Region/genetics Immunoglobulin M/analysis Immunoglobulin Variable Region/genetics Immunoglobulin kappa-Chains/genetics Molecular Sequence Data Mutation

Chemicals

Immunoglobulin D Immunoglobulin Joining Region Immunoglobulin M Immunoglobulin Variable Region Immunoglobulin kappa-Chains

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Klein U

Institute for Genetics, University of Cologne, FRG.

Küppers R

Rajewsky K

Article Info

Journal

European journal of immunology

Abbr.

Eur J Immunol

ISSN

0014-2980

DOI

10.1002/eji.1830231232

Published

1993-12-00

Pages

3272-7

Language

English

Region

Germany

NLM ID

1273201

Subset

Databases

GENBANK

Z26596, Z26597, Z26598, Z26599, Z26600, Z26601, Z26602, Z26603, Z26604, Z26605, Z26606, Z26607, Z26608, Z26609, Z26610, Z26611, Z26612, Z26613, Z26614, Z26615, Z26616, Z26617, Z26618, Z26619, Z26620, Z26621, Z26622, Z26623, Z26624, Z26625

External Links

PubMed source DOI: 10.1002/eji.1830231232 View journal details

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Human IgM+IgD+ B cells, the major B cell subset in the peripheral blood, express V kappa genes with no or little somatic mutation throughout life.

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