Home LiteratureArticle Details
PMID: 8253667 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A 3.9-kb DNA region of Xanthomonas campestris pv. campestris that is necessary for lipopolysaccharide production encodes a set of enzymes involved in the synthesis of dTDP-rhamnose.

Journal of bacteriology ·Vol. 175 ·No. 24 ·1993-12-00 ·Pages 7786-92

Köplin R, Wang G, Hötte B, Priefer UB, Pühler A

Abstract

By mutational analysis it was found that a 3.9-kb SmaI-XhoII DNA fragment of Xanthomonas campestris pv. campestris is involved in lipopolysaccharide (LPS) biosynthesis. LPS samples isolated from different mutants carrying mutations in the 3.9-kb SmaI-XhoII DNA fragment exhibited banding patterns in silver-stained sodium dodecyl sulfate-polyacrylamide gels markedly different from that of the wild-type LPS. Moreover, comparison of the monosaccharide composition obtained by high-performance anion-exchange chromatography with pulsed amperometric detection of LPS purified from wild-type Xanthomonas campestris pv. campestris B100 and from mutants with mutations in the 3.9-kb SmaI-XhoII DNA fragment revealed a lack of rhamnose moieties in the mutant LPS. Sequence analysis of this DNA fragment revealed four open reading frames (ORFs), designated ORF302, ORF183, ORF295, and ORF351. The deduced amino acid sequences of these ORFs showed a high degree of homology to the deduced amino acid sequences of the rfbC, rfbD, rfbA, and rfbB genes of Salmonella typhimurium LT2, which have been shown to encode a set of enzymes responsible for conversion of glucose 1-phosphate to dTDP-rhamnose.

Related Genes
rfbA rfbB rfbC rfbD
MeSH Terms
Amino Acid Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cloning, Molecular DNA Mutational Analysis DNA, Bacterial/genetics,metabolism Genes, Bacterial Lipopolysaccharides/chemistry,metabolism Molecular Sequence Data Monosaccharides/isolation & purification Nucleoside Diphosphate Sugars/biosynthesis Open Reading Frames Restriction Mapping Salmonella typhimurium/enzymology,genetics Sequence Homology, Amino Acid Thymine Nucleotides/biosynthesis Xanthomonas campestris/enzymology,genetics,metabolism
Chemicals
DNA, Bacterial Lipopolysaccharides Monosaccharides Nucleoside Diphosphate Sugars Thymine Nucleotides thymidine diphosphate rhamnose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Köplin R
Lehrstuhl für Genetik, Universität Bielefeld, Germany.
Wang G
Hötte B
Priefer U B
Pühler A
References (28)
28 references, click to expand
  1. Genetic and physical analyses of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris.
    J Bacteriol. 1987 Jun;169(6):2854-61 PMID: 3034868
  2. Electrophoretic separation of lipopolysaccharide monomers differing in polysaccharide length.
    Methods Enzymol. 1987;138:267-75 PMID: 2439873
  3. Evolution of Salmonella O antigen variation by interspecific gene transfer on a large scale.
    Trends Genet. 1993 Jan;9(1):17-22 PMID: 8434412
  4. Effects of galU mutation on flagellar formation in Escherichia coli.
    J Bacteriol. 1977 Feb;129(2):908-15 PMID: 320195
  5. The mechanism of 6-deoxyhexose synthesis. 3. Purification of deosythymidine diphosphate-glucose oxidoreductase.
    J Biol Chem. 1969 Sep 10;244(17):4750-6 PMID: 4309149
  6. Enzymatic synthesis and isolation of thymidine diphosphate-6-deoxy-D-xylo-4-hexulose and thymidine diphosphate-L-rhamnose. Production using cloned gene products and separation by HPLC.
    Eur J Biochem. 1992 Mar 1;204(2):539-45 PMID: 1541269
  7. The current status and portability of our sequence handling software.
    Nucleic Acids Res. 1986 Jan 10;14(1):217-31 PMID: 3511446
  8. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  9. CONTROL ASPECTS OF URIDINE 5'-DIPHOSPHATE GLUCOSE AND THYMIDINE 5'-DIPHOSPHATE GLUCOSE SYNTHESIS BY MICROBIAL ENZYMES.
    J Biol Chem. 1965 Jan;240:391-7 PMID: 14253441
  10. Cell wall lipopolysaccharides from xanthomonas species.
    J Bacteriol. 1966 Jan;91(1):39-42 PMID: 16562104
  11. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  12. Rhizobium leguminosarum CFN42 genetic regions encoding lipopolysaccharide structures essential for complete nodule development on bean plants.
    J Bacteriol. 1989 Jan;171(1):8-15 PMID: 2644215
  13. Clustering of mutations blocking synthesis of xanthan gum by Xanthomonas campestris.
    J Bacteriol. 1987 Aug;169(8):3593-600 PMID: 3038845
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
    J Bacteriol. 1990 May;172(5):2804-7 PMID: 2332409
  16. Comparison of bacterial lipopolysaccharides by high-performance liquid chromatography.
    Appl Environ Microbiol. 1986 Oct;52(4):948-50 PMID: 16347189
  17. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  18. Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae.
    J Mol Biol. 1988 Oct 5;203(3):715-38 PMID: 3062178
  19. Predicted nucleotide-binding properties of p21 protein and its cancer-associated variant.
    Nature. 1983 Apr 28;302(5911):842-4 PMID: 6843652
  20. Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Immunol Methods. 1990 Jan 24;126(1):109-17 PMID: 2106001
  21. Isolation of a bacterial lipopolysaccharide from Xanthomonas campestris containing 3-acetamido-3,6-dideoxy-D-galactose and D-rhamnose.
    J Biol Chem. 1966 Mar 25;241(6):1424-8 PMID: 5935353
  22. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains.
    J Bacteriol. 1983 Aug;155(2):831-8 PMID: 6409884
  23. Roles of flagella, lipopolysaccharide, and a Ca2+-dependent cell surface protein in attachment of Rhizobium leguminosarum biovar viciae to pea root hair tips.
    J Bacteriol. 1989 Jan;171(1):569-72 PMID: 2914856
  24. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  25. Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2).
    Mol Microbiol. 1991 Mar;5(3):695-713 PMID: 1710759
  26. Lipopolysaccharide-Defective Mutants of the Wilt Pathogen Pseudomonas solanacearum.
    Appl Environ Microbiol. 1984 Jul;48(1):94-101 PMID: 16346603
  27. Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis.
    J Bacteriol. 1992 Jan;174(1):191-9 PMID: 1370280
  28. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
    Anal Biochem. 1982 Jan 1;119(1):115-9 PMID: 6176137
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.24.7786-7792.1993
Published
1993-12-00
Pages
7786-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206953
Subset
IM
Databases
GENBANK
L23941
External Links
PubMed source DOI: 10.1128/jb.175.24.7786-7792.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2