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PMID: 8250554 Published · ppublish English Journal Article

Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis.

Applied and environmental microbiology ·Vol. 59 ·No. 10 ·1993-10-00 ·Pages 3273-9

Chang PK, Cary JW, Bhatnagar D, Cleveland TE, Bennett JW, Linz JE, Woloshuk CP, Payne GA

Abstract

An Aspergillus parasiticus gene, designated apa-2, was identified as a regulatory gene associated with aflatoxin biosynthesis. The apa-2 gene was cloned on the basis of overproduction of pathway intermediates following transformation of fungal strains with cosmid DNA containing the aflatoxin biosynthetic genes nor-1 and ver-1. Transformation of an O-methylsterigmatocystin-accumulating strain, A. parasiticus SRRC 2043, with a 5.5-kb HindIII-XbaI DNA fragment containing apa-2 resulted in overproduction of all aflatoxin pathway intermediates analyzed. Specific enzyme activities associated with the conversion of norsolorinic acid and sterigmatocystin were increased approximately twofold. The apa-2 gene was found to complement an A. flavus afl-2 mutant strain for aflatoxin production, suggesting that apa-2 is functionally homologous to afl-2. Comparison of the A. parasiticus apa-2 gene DNA sequence with that of the A. flavus afl-2 gene (G. A. Payne, G. J. Nystorm, D. Bhatnagar, T. E. Cleveland, and C. P. Woloshuk, Appl. Environ. Microbiol. 59:156-162, 1993) showed that they shared > 95% DNA homology. Physical mapping of cosmid subclones placed apa-2 approximately 8 kb from ver-1.

Related Genes
afl-2 apa-2
MeSH Terms
Aflatoxins/biosynthesis,genetics Aspergillus/drug effects,genetics,metabolism Base Sequence Chromosome Mapping Cloning, Molecular DNA Mutational Analysis DNA, Fungal/genetics Genes, Fungal Genes, Regulator Hot Temperature Molecular Sequence Data Nitrates/pharmacology Sequence Homology, Nucleic Acid Transformation, Genetic
Chemicals
Aflatoxins DNA, Fungal Nitrates
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chang P K
Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70118.
Cary J W
Bhatnagar D
Cleveland T E
Bennett J W
Linz J E
Woloshuk C P
Payne G A
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.59.10.3273-3279.1993
Published
1993-10-00
Pages
3273-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC182448
Subset
IM
Databases
GENBANK
L22177, S55473, X62979, X62980, X62981, X62986, X62987, X62988, X62989, X62990
External Links
PubMed source DOI: 10.1128/aem.59.10.3273-3279.1993 PMC Full Text View journal details
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