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PMID: 8244036 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two plasmids, X1918 and Z1918, for easy recovery of the xylE and lacZ reporter genes.

Gene ·Vol. 134 ·No. 1 ·1993-11-30 ·Pages 89-91

Schweizer HP

Abstract

Two plasmids, X1918 and Z1918, were constructed which contain the promoter-less xylE and lacZ reporter genes flanked symmetrically by the multiple cloning site (MCS) from pUC19. These cassettes can easily be derived from the multicopy plasmid, pUC1918.

Related Genes

cat lacZ xylE

MeSH Terms

Base Sequence Catechol 2,3-Dioxygenase Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Dioxygenases Genes, Reporter Molecular Sequence Data Mutagenesis, Insertional Oligodeoxyribonucleotides Oxygenases/genetics Plasmids Transcription, Genetic beta-Galactosidase/genetics

Chemicals

Oligodeoxyribonucleotides Oxygenases Dioxygenases Catechol 2,3-Dioxygenase Chloramphenicol O-Acetyltransferase beta-Galactosidase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Schweizer H P

Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Alberta, Canada.

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/0378-1119(93)90178-6

Published

1993-11-30

Pages

89-91

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Databases

GENBANK

L24529, U03991, U03992, U03993, U04610, X72987, X72988, X72989, Z15047, Z15048

External Links

PubMed source DOI: 10.1016/0378-1119(93)90178-6 View journal details

Analysis Services

Two plasmids, X1918 and Z1918, for easy recovery of the xylE and lacZ reporter genes.

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