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PMID: 8240335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The serum unresponsive Rous sarcoma virus promoter sustains a high serum response factor-dependent transcription in vitro.

Biochemical and biophysical research communications ·Vol. 196 ·No. 2 ·1993-10-29 ·Pages 611-8

Trouche D, Grigoriev M, Robin P, Harel-Bellan A

Abstract

CArG boxes are cis-regulatory elements which are represented both in serum responsive and unresponsive promoters. Here we show that the RSV Long Terminal Repeat contains two CArG boxes, which were efficiently recognised by purified Serum Response Factor, although they remained unresponsive to serum in transient transfection assays. However, RSV CArG boxes were as efficient as c-fos Serum Response Element in mediating a Serum Response Factor-dependent transcription in vitro. Thus, the fact that a CArG box is able to bind Serum Response Factor in an active form is insufficient for serum responsiveness in vivo.

Related Genes

Krox 20.1 Krox 20.2 c-fos

MeSH Terms

3T3 Cells Animals Avian Sarcoma Viruses/genetics Base Sequence Binding Sites DNA Primers DNA-Binding Proteins/metabolism Gene Expression Regulation, Viral Genes, fos Humans Leukemia, T-Cell Mice Molecular Sequence Data Nuclear Proteins/isolation & purification,metabolism Plasmids Polymerase Chain Reaction Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Restriction Mapping Serum Response Factor Transcription Factors/metabolism Transcription, Genetic Transfection Tumor Cells, Cultured

Chemicals

DNA Primers DNA-Binding Proteins Nuclear Proteins Serum Response Factor Transcription Factors

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Trouche D

Laboratoire de Biologie des Tumeurs Humaines, CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.

Grigoriev M

Robin P

Harel-Bellan A

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.1993.2293

Published

1993-10-29

Pages

611-8

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1006/bbrc.1993.2293 View journal details

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The serum unresponsive Rous sarcoma virus promoter sustains a high serum response factor-dependent transcription in vitro.

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