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PMID: 8240287 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Regulation of the glutamate transporter by amino acid deprivation and associated effects on the level of EAAC1 mRNA in the renal epithelial cell line NBL-I.

The Biochemical journal ·Vol. 295 ( Pt 3) ·1993-11-01 ·Pages 749-55

Plakidou-Dymock S, McGivan JD

Abstract

The glutamate transport system of the bovine renal epithelial cell line NBL-1 was studied. The Km for Na(+)-dependent glutamate transport was found to be 13.8 +/- 2.4 microM (Vmax. 365 +/- 19.2 pmol/3 min per mg) and for Na(+)-dependent aspartate transport 4.5 +/- 1.1 microM (Vmax. 108 +/- 6.3 pmol/3 min per mg). The Km values are in close agreement with those expected for high-affinity Na(+)-dependent glutamate transport by System XAG-. Upon deprivation of amino acids, the Vmax. for Na+/aspartate co-transport rose to 203 +/- 6.0 pmol/3 min per mg (Km 3.8 +/- 0.5 microns). A probe was constructed to the high-affinity excitatory amino acid carrier (EAAC1) [Kanai and Hediger (1992) Nature (London) 360, 467-471]. The probe hybridized to a 3.5 kb transcript. On deprivation of amino acids, the level of EAAC1 mRNA decreased sharply before the measurable increase in transport levels, but was subsequently restored to control levels. A motif, which we propose is linked to amino acid deprivation, was found in the EAAC1 primary sequence.

MeSH Terms
Amino Acid Sequence Amino Acid Transport System X-AG Amino Acids/administration & dosage Animals Aspartic Acid/metabolism Base Sequence Biological Transport Cattle Cell Line Epithelium/metabolism Glycoproteins/metabolism Kidney/metabolism Kinetics Molecular Sequence Data Nucleic Acid Hybridization RNA, Messenger/metabolism Sodium/pharmacology
Chemicals
Amino Acid Transport System X-AG Amino Acids Glycoproteins RNA, Messenger Aspartic Acid Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Plakidou-Dymock S
Department of Biochemistry, University of Bristol, School of Medical Sciences, U.K.
McGivan J D
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2950749
Published
1993-11-01
Pages
749-55
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1134624
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj2950749 PMC Full Text View journal details
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