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PMID: 8233816 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conformational analysis of the 5' leader and the gag initiation site of Mo-MuLV RNA and allosteric transitions induced by dimerization.

Nucleic acids research ·Vol. 21 ·No. 20 ·1993-10-11 ·Pages 4677-84

Mougel M, Tounekti N, Darlix JL, Paoletti J, Ehresmann B, Ehresmann C

Abstract

Dimerization of genomic RNA is a key step in the retroviral life cycle and has been postulated to be involved in the regulation of translation, encapsidation and reverse transcription. Here, we have derived a secondary structure model of nucleotides upstream from psi and of the gag initiation region of Mo-MuLV RNA in monomeric and dimeric forms, using chemical probing, sequence comparison and computer prediction. The 5' domain is extensively base-paired and interactions take place between U5 and 5' leader sequences. The U5-PBS subdomain can fold in two mutually exclusive conformations: a very stable and extended helical structure (E form) in which 17 of the 18 nucleotides of the PBS are paired, or an irregular three-branch structure (B form) in which 10 nucleotides of the PBS are paired. The dimeric RNA adopts the B conformation. The monomeric RNA can switch from the E to the B conformation by a thermal treatment. If the E to B transition is associated to dimerization, it may facilitate annealing of the primer tRNAPro to the PBS by lowering the free energy required for melting the PBS. Furthermore, dimerization induces allosteric rearrangements around the SD site and the gag initiation region.

Related Genes
gag
MeSH Terms
Allosteric Regulation Base Sequence Biopolymers Genes, gag Molecular Sequence Data Moloney murine leukemia virus/genetics Nucleic Acid Conformation RNA, Viral/chemistry,genetics Sequence Homology, Nucleic Acid
Chemicals
Biopolymers RNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mougel M
UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Tounekti N
Darlix J L
Paoletti J
Ehresmann B
Ehresmann C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.20.4677
Published
1993-10-11
Pages
4677-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331490
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/21.20.4677 PMC Full Text View journal details
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