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PMID: 8226719 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

NAD(P)H-ubiquinone oxidoreductases in plant mitochondria.

Journal of bioenergetics and biomembranes ·Vol. 25 ·No. 4 ·1993-08-00 ·Pages 377-84

Møller IM, Rasmusson AG, Fredlund KM

Abstract

Plant (and fungal) mitochondria contain multiple NAD(P)H dehydrogenases in the inner membrane all of which are connected to the respiratory chain via ubiquinone. On the outer surface, facing the intermembrane space and the cytoplasm, NADH and NADPH are oxidized by what is probably a single low-molecular-weight, nonproton-pumping, unspecific rotenone-insensitive NAD(P)H dehydrogenase. Exogenous NADH oxidation is completely dependent on the presence of free Ca2+ with a K0.5 of about 1 microM. On the inner surface facing the matrix there are two dehydrogenases: (1) the proton-pumping rotenone-sensitive multisubunit Complex I with properties similar to those of Complex I in mammalian and fungal mitochondria. (2) a rotenone-insensitive NAD(P)H dehydrogenase with equal activity with NADH and NADPH and no proton-pumping activity. The NADPH-oxidizing activity of this enzyme is completely dependent on Ca2+ with a K0.5 of 3 microM. The enzyme consists of a single subunit of 26 kDa and has a native size of 76 kDa, which means that it may form a trimer.

MeSH Terms
Calcium/physiology Electron Transport Fungal Proteins/chemistry,metabolism Membrane Proteins/chemistry,metabolism Mitochondria/enzymology NAD/metabolism NAD(P)H Dehydrogenase (Quinone)/antagonists & inhibitors,chemistry,isolation & purification,metabolism NADP/metabolism NADPH Dehydrogenase/isolation & purification,metabolism Oxidation-Reduction Plant Proteins/chemistry,isolation & purification,metabolism Protein Conformation Protons Rotenone/pharmacology
Chemicals
Fungal Proteins Membrane Proteins Plant Proteins Protons Rotenone NAD NADP NAD(P)H Dehydrogenase (Quinone) NADPH Dehydrogenase Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Møller I M
Department of Plant Biology, Lund University, Sweden.
Rasmusson A G
Fredlund K M
References (50)
50 references, click to expand
  1. The external NADH dehydrogenases of intact plant mitochondria.
    Biochim Biophys Acta. 1973 Jan 18;292(1):105-16 PMID: 4145130
  2. RNA editing at a splicing site of NADH dehydrogenase subunit IV gene transcript in wheat mitochondria.
    FEBS Lett. 1989 Nov 20;258(1):79-83 PMID: 2687023
  3. Ca2+ stimulation of the external NADH dehydrogenase in Jerusalem artichoke (Helianthus tuberosum) mitochondria.
    Biochem Biophys Res Commun. 1982 Nov 30;109(2):513-7 PMID: 7181932
  4. The regulation and nature of the cyanide-resistant alternative oxidase of plant mitochondria.
    Biochim Biophys Acta. 1991 Aug 23;1059(2):121-40 PMID: 1883834
  5. Expression of the wheat mitochondrial nad3-rps12 transcription unit: correlation between editing and mRNA maturation.
    Plant Cell. 1991 Oct;3(10):1109-20 PMID: 1726558
  6. The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are co-transcribed.
    Mol Gen Genet. 1988 Dec;215(1):118-27 PMID: 2853827
  7. The wheat mitochondrial genome contains an ORF showing sequence homology to the gene encoding the subunit 6 of the NADH-ubiquinone oxidoreductase.
    Plant Mol Biol. 1992 Nov;20(3):395-404 PMID: 1421143
  8. Purification and characterization of the rotenone-insensitive NADH dehydrogenase of mitochondria from Arum maculatum.
    Eur J Biochem. 1984 Jun 15;141(3):573-7 PMID: 6745260
  9. Ionic strength of the intermembrane space of intact mitochondria as estimated with fluorescein-BSA delivered by low pH fusion.
    J Cell Biol. 1991 Jun;113(6):1331-40 PMID: 2045415
  10. Purification and Partial Characterization of Two Soluble NAD(P)H Dehydrogenases from Arum maculatum Mitochondria.
    Plant Physiol. 1991 Mar;95(3):934-42 PMID: 16668075
  11. Purification and characterization of a rotenone-insensitive NADH:Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae.
    Eur J Biochem. 1988 Sep 15;176(2):377-84 PMID: 3138118
  12. Oxidation of External NAD(P)H by Jerusalem Artichoke (Helianthus tuberosus) Mitochondria : A Kinetic and Inhibitor Study.
    Plant Physiol. 1992 Jul;99(3):1037-43 PMID: 16668968
  13. The stimulation of exogenous NADH oxidation in Jerusalem artichoke mitochondria by screening of charges on the membranes.
    FEBS Lett. 1979 Dec 1;108(1):28-32 PMID: 230083
  14. Partial Purification and Characterization of Complex I, NADH:Ubiquinone Reductase, from the Inner Membrane of Beetroot Mitochondria.
    Plant Physiol. 1992 Feb;98(2):588-94 PMID: 16668682
  15. Interactions of reduced and oxidized triphosphopyridine nucleotides with the electron-transport system of bovine heart mitochondria.
    Biochemistry. 1973 Aug 28;12(18):3515-22 PMID: 4147216
  16. Matrix NADH dehydrogenases of plant mitochondria and sites of quinone reduction by complex I.
    Eur J Biochem. 1992 Sep 1;208(2):481-5 PMID: 1521539
  17. Oxidation of reduced nicotinamide adenine dinucleotide phosphate by plant mitochondria.
    Can J Biochem. 1979 Dec;57(12):1392-9 PMID: 44218
  18. Oxidation of Reduced Nicotinamide Adenine Dinucleotide Phosphate by Potato Mitochondria: INHIBITION BY SULFHYDRYL REAGENTS.
    Plant Physiol. 1980 Apr;65(4):591-4 PMID: 16661244
  19. Electron paramagnetic resonance characterization of membrane bound iron-sulfur clusters and aconitase in plant mitochondria.
    Plant Physiol. 1986 May;81(1):247-52 PMID: 16664783
  20. Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae.
    Eur J Biochem. 1992 Feb 1;203(3):587-92 PMID: 1735444
  21. Regulation of malate oxidation in plant mitochondria. Response to rotenone and exogenous NAD+.
    Biochem J. 1982 Dec 15;208(3):703-11 PMID: 6819864
  22. NADP-Utilizing Enzymes in the Matrix of Plant Mitochondria.
    Plant Physiol. 1990 Nov;94(3):1012-8 PMID: 16667790
  23. Mitochondria from the Mesophyll Cells of Zea mays Leaves.
    J Plant Physiol. 1984;116(4):351-64 PMID: 23195305
  24. The regulation of exogenous NAD(P)H oxidation in spinach (Spinacia oleracea) leaf mitochondria by pH and cations.
    Biochem J. 1985 Dec 1;232(2):471-7 PMID: 3937519
  25. The bacterial energy-transducing NADH-quinone oxidoreductases.
    Biochim Biophys Acta. 1993 Feb 8;1141(1):1-17 PMID: 8435434
  26. Separation Procedure and Partial Characterization of Two NAD(P)H Dehydrogenases from Cauliflower Mitochondria.
    Plant Physiol. 1984 Oct;76(2):436-41 PMID: 16663860
  27. Analysis of NADH dehydrogenases from plant [mung bean (Phaseolus aureus)] mitochondrial membranes on non-denaturing polyacrylamide gels and purification of complex I by band excision.
    Biochem J. 1988 Aug 15;254(1):303-5 PMID: 3178753
  28. Partial purification and properties of the external NADH dehydrogenase from cuckoo-pint (Arum maculatum) mitochondria.
    Biochem J. 1984 Nov 15;224(1):171-9 PMID: 6508755
  29. The watermelon mitochondrial URF-1 gene: evidence for a complex structure.
    Curr Genet. 1986;10(11):857-69 PMID: 3447741
  30. Partial Purification and Characterization of Three NAD(P)H Dehydrogenases from Beta vulgaris Mitochondria.
    Plant Physiol. 1991 Dec;97(4):1317-22 PMID: 16668549
  31. Sugarbeet mitochondria contain an open reading frame showing extensive sequence homology to the subunit 2 gene of the NADH: ubiquinone reductase complex.
    Mol Gen Genet. 1990 Apr;221(2):195-8 PMID: 2115110
  32. Partial characterization of the gene coding for subunit IV of soybean mitochondrial NADH dehydrogenase.
    Curr Genet. 1989 Feb;15(2):155-60 PMID: 2743433
  33. Effects of Polyamines on the Oxidation of Exogenous NADH by Jerusalem Artichoke (Helianthus tuberosus) Mitochondria.
    Plant Physiol. 1991 Jan;95(1):157-63 PMID: 16667944
  34. A Comparison between Quin-2 and Aequorin as Indicators of Cytoplasmic Calcium Levels in Higher Plant Cell Protoplasts.
    Plant Physiol. 1989 Jun;90(2):482-91 PMID: 16666797
  35. The respiratory-chain NADH dehydrogenase (complex I) of mitochondria.
    Eur J Biochem. 1991 May 8;197(3):563-76 PMID: 2029890
  36. Effect of calcium ions and inhibitors on internal NAD(P)H dehydrogenases in plant mitochondria.
    Eur J Biochem. 1991 Dec 5;202(2):617-23 PMID: 1722151
  37. Charge screening by cations affects the conformation of the mitochondrial inner membrane. A study of exogenous MAD(P)H oxidation in plant mitochondria.
    Biochem J. 1981 Jun 1;195(3):583-8 PMID: 7316973
  38. Electrostatic screening stimulates rate-limiting steps in mitochondrial electron transport.
    Biochem J. 1984 Nov 1;223(3):761-7 PMID: 6095808
  39. Pathways of hydrogen in mitochondria of Saccharomyces carlsbergensis.
    Eur J Biochem. 1970 Feb;12(3):583-92 PMID: 4314881
  40. EPR studies of iron-sulphur proteins of plant mitochondria.
    Ann N Y Acad Sci. 1973 Dec 31;222:816-23 PMID: 4361882
  41. Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae.
    Eur J Biochem. 1991 Feb 14;195(3):857-62 PMID: 1900238
  42. 9-Aminoacridine as a fluorescent probe of the electrical diffuse layer associated with the membranes of plant mitochondria.
    Biochem J. 1981 Jan 1;193(1):37-46 PMID: 7305932
  43. A specific role for Ca2+ in the oxidation of exogenous NADH by Jerusalem-artichoke (Helianthus tuberosus) mitochondria.
    Biochem J. 1981 Feb 15;194(2):487-95 PMID: 6796061
  44. Oxidation of External NAD(P)H by Purified Mitochondria from Fresh and Aged Red Beetroots (Beta vulgaris L.).
    Plant Physiol. 1991 Sep;97(1):99-103 PMID: 16668422
  45. Characteristics of External NADH Oxidation by Beetroot Mitochondria.
    Plant Physiol. 1976 Jul;58(1):38-42 PMID: 16659616
  46. Oxidation of reduced nicotinamide adenine dinucleotide phosphate by isolated corn mitochondria.
    Plant Physiol. 1972 Mar;49(3):353-7 PMID: 16657960
  47. The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains two introns and is co-transcribed with the 5 S rRNA gene.
    Mol Gen Genet. 1988 Apr;212(1):56-65 PMID: 3163767
  48. Immunological analysis of plant mitochondrial NADH dehydrogenases.
    Biochem J. 1986 May 15;236(1):201-7 PMID: 3790070
  49. Bacterial NADH-quinone oxidoreductases.
    J Bioenerg Biomembr. 1991 Apr;23(2):211-25 PMID: 2050655
  50. Binding and screening by cations and the effect on exogenous NAD(P)H oxidation in Neurospora crassa mitochondria.
    Eur J Biochem. 1982 Mar;123(1):81-8 PMID: 6461553
Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00762463
Published
1993-08-00
Pages
377-84
Language
English
Region
United States
NLM ID
7701859
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00762463 View journal details
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