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PMID: 8226633 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

SKN7, a yeast multicopy suppressor of a mutation affecting cell wall beta-glucan assembly, encodes a product with domains homologous to prokaryotic two-component regulators and to heat shock transcription factors.

Journal of bacteriology ·Vol. 175 ·No. 21 ·1993-11-00 ·Pages 6908-15

Brown JL, North S, Bussey H

Abstract

A search for genes which, at elevated copy number, could suppress the growth defect in a strain disrupted at the KRE9 locus has identified the SKN7 gene. SKN7 was mapped to the right arm of chromosome VIII and is predicted to encode a 70-kDa protein, Skn7p, with a region of homology to the DNA binding domain of the Saccharomyces cerevisiae heat shock transcription factor, Hsf1p. Skn7p also has a domain which shows similarity to the prokaryotic receiver modules found on an extensive family of two-component response regulators, including the products of the rcsC and barA genes. SKN7 did not suppress other mutations in the (1-->6)-beta-glucan biosynthetic pathway, suggesting that SKN7 does not act as a general bypass suppressor of this glucan.

Related Genes
KRE9 SKN7 barA resC
MeSH Terms
Amino Acid Sequence Base Sequence Cell Wall/metabolism DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Genes, Suppressor Glucans/biosynthesis Heat-Shock Proteins/genetics Molecular Sequence Data Mutagenesis Restriction Mapping Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Deletion Sequence Homology, Amino Acid Suppression, Genetic Transcription Factors/genetics,metabolism
Chemicals
DNA-Binding Proteins Glucans Heat-Shock Proteins SKN7 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brown J L
Biology Department, McGill University, Montreal, Quebec, Canada.
North S
Bussey H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.21.6908-6915.1993
Published
1993-11-00
Pages
6908-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206816
Subset
IM
Databases
GENBANK
U00485
External Links
PubMed source DOI: 10.1128/jb.175.21.6908-6915.1993 PMC Full Text View journal details
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