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PMID: 8226625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mycobacteriophage L5 integrase-mediated site-specific integration in vitro.

Journal of bacteriology ·Vol. 175 ·No. 21 ·1993-11-00 ·Pages 6836-41

Lee MH, Hatfull GF

Abstract

Mycobacteriophage L5, a temperate phage of the mycobacteria, forms stable lysogens in Mycobacterium smegmatis via site-specific integration of the phage genome. Recombination occurs within specific phage and bacterial attachment sites and is catalyzed by the phage-encoded integrase protein in vivo. We describe here the overexpression and purification of L5 integrase and its ability to mediate integrative recombination in vitro. We find that L5 integrase-mediated recombination is greatly stimulated by extracts of M. smegmatis but not by Escherichia coli extracts, purified E. coli integration host factor, or purified HU, indicating the presence of a novel mycobacterial integration host factor.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Nucleotidyltransferases/biosynthesis,isolation & purification,metabolism DNA Primers Integrases Kinetics Molecular Sequence Data Mycobacteriophages/enzymology,genetics Mycobacterium/enzymology,genetics Plasmids Recombinant Proteins/biosynthesis,isolation & purification,metabolism Recombination, Genetic Restriction Mapping Thermodynamics Virus Integration
Chemicals
DNA Primers Recombinant Proteins DNA Nucleotidyltransferases Integrases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee M H
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Hatfull G F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.21.6836-6841.1993
Published
1993-11-00
Pages
6836-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206807
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.21.6836-6841.1993 PMC Full Text View journal details
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