Preparation of red cells coated with C4 and C3 subcomponents and production of anti-C4d and anti-C3d.

Vox sanguinis ·Vol. 31 ·No. 4 ·1976-10-00 ·Pages 241-57

Freedman J, Mollison PL

Abstract

Methods of preparing red cells coated only with C4b, C3b or C3d have previously been described by others; the present observations supply further information about the optimal conditions for preparing such cells and also describe conditions for the operation of cells carrying only C4d. In addition, the preparation of specific anti-C4d and anti-C3d sera is described.

MeSH Terms

Animals Antibody Formation Antigen-Antibody Reactions Calcium/metabolism Cations, Divalent Complement C3/metabolism Complement C4/metabolism Complement Fixation Tests/methods Complement System Proteins/metabolism Edetic Acid/pharmacology Erythrocytes/metabolism Humans Immune Sera/isolation & purification Magnesium/metabolism Rabbits Sheep Sucrose/metabolism Time Factors Trypsin/pharmacology

Chemicals

Cations, Divalent Complement C3 Complement C4 Immune Sera Sucrose Complement System Proteins Edetic Acid Trypsin Magnesium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Freedman J

Mollison P L

Article Info

Journal

Vox sanguinis

Abbr.

Vox Sang

ISSN

0042-9007

DOI

10.1111/j.1423-0410.1976.tb04647.x

Published

1976-10-00

Pages

241-57

Language

English

Region

England

NLM ID

0413606

Subset

External Links

PubMed source DOI: 10.1111/j.1423-0410.1976.tb04647.x View journal details

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Preparation of red cells coated with C4 and C3 subcomponents and production of anti-C4d and anti-C3d.

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