Determination of molecular weights by fluctuation spectroscopy: application to DNA.
A method for determining molecular weights of macromolecules by measuring spontaneous concentration fluctuations is described. The method is absolute, rapid, and requires no shearing forces on the molecules. We have applied this technique to the determination of molecular weight of DNA molecules. The molecular weight values obtained for T2 phage DNA (1.14 X 10(8)) and replicating Escherichia coli DNA (3.9 X 10(9)) agree with previous results. By monitoring individual molecules, an estimate of the molecular weight of nuclei and individual chromosomal DNA molecules of Drosophila melanogaster was obtained.
18 references, click to expand
-
Physical studies on DNA from "primitive" eucaryotes.CRC Crit Rev Biochem. 1975;3(4):349-449 PMID: 819212
-
Automated analysis of deoxyribonucleic acid, protein and nuclear to cytoplasmic relationships in tumor cells and gynecologic specimens.J Histochem Cytochem. 1974 Jul;22(7):616-21 PMID: 4136550
-
Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition.J Bacteriol. 1974 Dec;120(3):1356-63 PMID: 4612018
-
Chromosome-sized DNA molecules from Drosophila.Chromosoma. 1973;41(1):1-27 PMID: 4632500
-
Fluorescence correlation spectroscopy. II. An experimental realization.Biopolymers. 1974 Jan;13(1):29-61 PMID: 4818131
-
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.J Mol Biol. 1970 Dec 28;54(3):567-77 PMID: 4923669
-
Viscosity and sedimentation of the DNA from bacteriophages T2 and T7 and the relation to molecular weight.J Mol Biol. 1965 Jul;12(3):525-36 PMID: 5858429
-
Complex formation between ethidium bromide and nucleic acids.J Mol Biol. 1965 Aug;13(1):269-82 PMID: 5859041
-
The molecular weights of T2 bacteriophage DNA and its first and second breakage products.Proc Natl Acad Sci U S A. 1961 Aug;47:1113-22 PMID: 13744329
-
Fluctuation spectroscopy: determination of chemical reaction kinetics from the frequency spectrum of fluctuations.Proc Natl Acad Sci U S A. 1973 Mar;70(3):870-5 PMID: 16592071
-
DNA polyintercalating drugs: DNA binding of diacridine derivatives.Proc Natl Acad Sci U S A. 1975 Aug;72(8):2915-9 PMID: 127172
-
Diacridines: bifunctional intercalators. I. Chemistry, physical chemistry and growth inhibitory properties.Biochim Biophys Acta. 1976 Feb 5;418(3):277-89 PMID: 1247545
-
The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.J Mol Biol. 1968 Apr 14;33(1):141-71 PMID: 4296517
-
Size of DNA determined by viscoelastic measurements: results on bacteriophages, Bacillus subtilis and Escherichia coli.J Mol Biol. 1972 Dec 30;72(3):779-800 PMID: 4633907
-
Chromosome replication and the division cycle of Escherichia coli B/r.J Mol Biol. 1968 Feb 14;31(3):519-40 PMID: 4866337
-
The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis.Genetics. 1965 Oct;52(4):747-57 PMID: 4953222
-
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.J Mol Biol. 1967 Jul 14;27(1):87-106 PMID: 6033613
-
THE USE OF HOT PHENOL IN PREPARING DNA.Proc Natl Acad Sci U S A. 1965 Dec;54(6):1641-3 PMID: 16591324
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