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PMID: 821920 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Low-frequency, pbsi-mediated plasmid transduction in Bacillus pumilus.

Journal of bacteriology ·Vol. 127 ·No. 2 ·1976-08-00 ·Pages 829-31

Bramucci MG, Lovett PS

Abstract

Three bacteriophages were tested for ability to transduce the plasmid of pPL10 between W mutant derivatives of Bacillus pumilus NRS 576. Phage PBP1- and PMB1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. Phage PBS1-generated plasmid transductants occurred at less than 0.1% the frequency of transductants for a chromosome marker. Possible reasons for the extremely reduced capacity of PBS1 to generate plasmid transductants are discussed.

MeSH Terms
Bacillus/analysis Bacillus subtilis Bacteriophages/growth & development DNA, Bacterial/analysis DNA, Circular/analysis Extrachromosomal Inheritance Mutation Plasmids Transduction, Genetic
Chemicals
DNA, Bacterial DNA, Circular
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bramucci M G
Lovett P S
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.127.2.829-831.1976
Published
1976-08-00
Pages
829-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC232990
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.127.2.829-831.1976 PMC Full Text View journal details
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