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PMID: 8206862 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Expression of a yeast intron-containing tRNA in the archaeon Haloferax volcanii.

Journal of bacteriology ·Vol. 176 ·No. 12 ·1994-06-00 ·Pages 3820-3

Palmer JR, Nieuwlandt DT, Daniels CJ

Abstract

Expression of the yeast tRNAPro(UGG) gene in Haloferax volcanii resulted in the production of a single stable transcript that had not undergone intron processing or processing of 5' and 3' flanking sequences. Mutation of the exon-intron boundary region of this RNA to produce a precursor RNA with the preferred halobacterial consensus exon-intron boundary structure did not restore intron processing. Processing of 5' and 3' flanking sequences was restored when the acceptor stem U6-U67 pair was changed to A6-U67. The significance of these results in defining the recognition requirements of tRNA maturation enzymes in the halophilic domain Archaea is discussed.

MeSH Terms
Base Sequence Halobacteriaceae/genetics,metabolism Introns/genetics Molecular Sequence Data Nucleic Acid Conformation RNA Processing, Post-Transcriptional RNA, Transfer, Pro/biosynthesis,genetics Saccharomyces cerevisiae/genetics
Chemicals
RNA, Transfer, Pro
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Palmer J R
Department of Microbiology, Ohio State University, Columbus 43210.
Nieuwlandt D T
Daniels C J
References (20)
20 references, click to expand
  1. Comparison of transfer RNA and ribosomal RNA intron splicing in the extreme thermophile and archaebacterium Desulfurococcus mobilis.
    Can J Microbiol. 1989 Jan;35(1):210-4 PMID: 2470485
  2. Presence of an intron in elongator methionine-tRNA of Halobacterium volcanii.
    Can J Microbiol. 1989 Jan;35(1):189-94 PMID: 2470482
  3. Recognition of exon-intron boundaries by the Halobacterium volcanii tRNA intron endonuclease.
    J Biol Chem. 1990 Oct 25;265(30):18104-11 PMID: 1698785
  4. An expression vector for the archaebacterium Haloferax volcanii.
    J Bacteriol. 1990 Dec;172(12):7104-10 PMID: 1701429
  5. The RNA component of RNase P from the archaebacterium Haloferax volcanii.
    J Biol Chem. 1991 Mar 25;266(9):5689-95 PMID: 1706337
  6. Six group I introns and three internal transcribed spacers in the chloroplast large subunit ribosomal RNA gene of the green alga Chlamydomonas eugametos.
    J Mol Biol. 1991 Mar 20;218(2):293-311 PMID: 1849178
  7. The acceptor stem in pre-tRNAs determines the cleavage specificity of RNase P.
    Nucleic Acids Res. 1992 Feb 11;20(3):421-3 PMID: 1371348
  8. Exon sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.
    Biochemistry. 1992 Mar 3;31(8):2359-67 PMID: 1540592
  9. Participation of the intron in the reaction catalyzed by the Xenopus tRNA splicing endonuclease.
    Science. 1992 Mar 13;255(5050):1404-8 PMID: 1542788
  10. Protein-coding introns from the 23S rRNA-encoding gene form stable circles in the hyperthermophilic archaeon Pyrobaculum organotrophum.
    Gene. 1992 Nov 2;121(1):103-10 PMID: 1427083
  11. Recent studies of ribonuclease P.
    FASEB J. 1993 Jan;7(1):7-14 PMID: 7916700
  12. In vivo processing of an intron-containing archael tRNA.
    Mol Microbiol. 1993 Apr;8(1):93-9 PMID: 7684487
  13. In vitro genetic analysis of the structural features of the pre-tRNA required for determination of the 3' splice site in the intron excision reaction.
    EMBO J. 1993 Dec;12(12):4697-704 PMID: 8223479
  14. Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.
    Nucleic Acids Res. 1984 Dec 21;12(24):9367-82 PMID: 6096826
  15. Transcription and excision of a large intron in the tRNATrp gene of an archaebacterium, Halobacterium volcanii.
    J Biol Chem. 1985 Mar 10;260(5):3132-4 PMID: 3844407
  16. Characteristics of ribonuclease P from various organisms.
    Cold Spring Harb Symp Quant Biol. 1987;52:233-8 PMID: 2456877
  17. Substrate recognition and splice site determination in yeast tRNA splicing.
    Cell. 1988 Nov 18;55(4):719-30 PMID: 3141064
  18. Site selection by the tRNA splicing endonuclease of Xenopus laevis.
    Cell. 1988 Nov 18;55(4):731-8 PMID: 3180224
  19. A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties.
    J Biol Chem. 1988 Dec 5;263(34):17951-9 PMID: 3192521
  20. Transfer RNA intron processing in the halophilic archaebacteria.
    Can J Microbiol. 1989 Jan;35(1):36-42 PMID: 2470486
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.12.3820-3823.1994
Published
1994-06-00
Pages
3820-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205573
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.12.3820-3823.1994 PMC Full Text View journal details
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