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PMID: 8205352 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunohistochemical evaluation of alpha-catenin expression in human gastric cancer.

Virchows Archiv : an international journal of pathology ·Vol. 424 ·No. 4 ·1994-00-00 ·Pages 375-81

Matsui S, Shiozaki H, Inoue M, Tamura S, Doki Y, Kadowaki T, Iwazawa T, Shimaya K, Nagafuchi A, Tsukita S

Abstract

E-cadherin (E-cad) plays a major role in the maintenance of cell-cell adhesion in epithelial tissues, and impaired E-cad expression correlates with tumour invasion and metastasis. Alpha-catenin (alpha-cat), an undercoat protein of adherens junctions, binds to the cytoplasmic domain of E-cad and is essential for linking E-cad to actin-based cytoskeleton. We investigated E-cad and alpha-cat expression in 60 human gastric cancers immunohistochemically. The 60 gastric cancers were classified into 18 (30%) in which alpha-cat expression was preserved, and 42 (70%) reduced cases. The reduction of alpha-cat expression was significantly related to dedifferentiation, depth of invasion, infiltrative growth and lymph node metastasis. We also examined the co-expression of alpha-cat and E-cad. Seventeen (28%) tumours preserved both molecules [alpha-cat(+)/E-cad(+)] and 33 (55%) tumours reduced both [alpha-cat(-)/E-cad(-)], whereas 9 (15%) tumours exhibited alpha-cat(-)/E-cad(+). The frequency of lymph node metastasis in alpha-cat(-)/E-cad(+) tumour (67%) was significantly higher than that in alpha-cat(+)/E-cad(+) tumours (24%) and was close to that in alpha-cat(-)/E-cad(-) tumours (82%). The frequency of haematogenous liver metastasis in alpha-cat(-)/E-cad(+) tumours (44%) was significantly higher than that in alpha-cat(+)/E-cad(+) tumours (6%) or alpha-cat(-)/E-cad(-) tumours (9%). Thus, in all E-cad(+) tumours, the frequency of lymph node and liver metastasis was higher in alpha-cat(-) tumours than in alpha-cat(+) tumours. alpha-Cat expression is apparently better at predicting tumour invasion and metastasis than E-cad expression.

MeSH Terms
Adult Aged Cadherins/metabolism Cytoskeletal Proteins/metabolism Humans Immunohistochemistry Middle Aged Neoplasm Invasiveness Neoplasm Metastasis Stomach Neoplasms/metabolism,pathology alpha Catenin
Chemicals
CTNNA1 protein, human Cadherins Cytoskeletal Proteins alpha Catenin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Matsui S
Department of Surgery II, Osaka University Medical School, Japan.
Shiozaki H
Inoue M
Tamura S
Doki Y
Kadowaki T
Iwazawa T
Shimaya K
Nagafuchi A
Tsukita S
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Article Info
Journal
Virchows Archiv : an international journal of pathology
Abbr.
Virchows Arch
ISSN
0945-6317
DOI
10.1007/BF00190559
Published
1994-00-00
Pages
375-81
Language
English
Region
Germany
NLM ID
9423843
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00190559 View journal details
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