Purification of genomic DNA from human whole blood by isopropanol-fractionation with concentrated Nal and SDS.

Nucleic acids research ·Vol. 22 ·No. 9 ·1994-05-11 ·Pages 1774-5

Wang L, Hirayasu K, Ishizawa M, Kobayashi Y

Abstract

暂无摘要

MeSH Terms

1-Propanol DNA/blood,isolation & purification Electrophoresis, Agar Gel Humans Polymerase Chain Reaction Sodium Dodecyl Sulfate Sodium Iodide

Chemicals

Sodium Dodecyl Sulfate DNA 1-Propanol Sodium Iodide

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wang L

Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd., Hyogo, Japan.

Hirayasu K

Ishizawa M

Kobayashi Y

References (6)

6 references, click to expand

A general method for isolation of high molecular weight DNA from eukaryotes.
Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
Purification of cloned and genomic DNA by guanidine thiocyanate/isobutyl alcohol fractionation.
Anal Biochem. 1992 Nov 15;207(1):197-201 PMID: 1489096
Simple procedure of DNA isolation from human serum.
Nucleic Acids Res. 1991 Oct 25;19(20):5792 PMID: 1945860
Purification of human leucocyte DNA: proteinase K is not necessary.
Anal Biochem. 1992 Mar;201(2):362-5 PMID: 1632526
A simple salting out procedure for extracting DNA from human nucleated cells.
Nucleic Acids Res. 1988 Feb 11;16(3):1215 PMID: 3344216

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1994-05-11

Pages

1774-5

Language

English

Region

England

NLM ID

0411011

PMCID

PMC308067

Subset

External Links

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