Effect of sonic treatment on pure cultures and aggregates of bacteria.

Journal of clinical microbiology ·Vol. 3 ·No. 5 ·1976-05-00 ·Pages 474-9

Robrish SA, Grove SB, Bernstein RS, Marucha PT, Socransky SS, Amdur B

Abstract

Pure cultures of a variety of bacteria were treated with ultrasonic energy using a sonic probe. Fractions of organisms killed at different sonic energies were calculated, and Streptococcus mutans was 600 times more resistant than Fusobacterium nucleatum, the most sensitive organism tested. The effects of sonic treatment on aggregates of bacteria were examined, and the results were interpreted as a model of the events that probably occur during the sonic dispersion of dental plaque.

MeSH Terms

Actinomyces/cytology Bacteria/cytology,metabolism Bacteroides fragilis/cytology Cell Aggregation Cell Count Cell Survival Dental Plaque/microbiology Escherichia coli/cytology Fusobacterium/cytology Humans Lactobacillus casei/cytology Neisseria/cytology Polysaccharides, Bacterial/biosynthesis Streptococcus mutans/cytology Ultrasonics Veillonella/cytology

Chemicals

Polysaccharides, Bacterial

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Robrish S A

Grove S B

Bernstein R S

Marucha P T

Socransky S S

Amdur B

References (6)

6 references, click to expand

ULTRASTRUCTURE OF VEILLONELLA AND MORPHOLOGICAL CORRELATION OF AN OUTER MEMBRANE WITH PARTICLES ASSOCIATED WITH ENDOTOXIC ACTIVITY.
J Bacteriol. 1964 Nov;88:1482-92 PMID: 14234809
Observations on the use of ultrasound waves for the disruption of micro-organisms.
Biochim Biophys Acta. 1959 Jun;33(2):481-93 PMID: 13670919
Sonic disruption of Azotobacter vinelandil.
J Bacteriol. 1957 Jul;74(1):79-86 PMID: 13462964
Effects of sonic vibration on the numbers of microorganisms cultivable from human saliva.
J Dent Res. 1952 Jun;31(3):428-39 PMID: 14938501
Relationship of bacteria to the etiology of periodontal disease.
J Dent Res. 1970 Mar-Apr;49(2):203-22 PMID: 4313844
The oral microbiota of man from birth to senility.
J Periodontol. 1971 Aug;42(8):485-96 PMID: 4998039

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.3.5.474-479.1976

Published

1976-05-00

Pages

474-9

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC274334

Subset

External Links

PubMed source DOI: 10.1128/jcm.3.5.474-479.1976 PMC Full Text View journal details

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Effect of sonic treatment on pure cultures and aggregates of bacteria.

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