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PMID: 8190070 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A gene encoding a novel isoform of the PR-1 protein family from tomato is induced upon viroid infection.

Molecular & general genetics : MGG ·Vol. 243 ·No. 1 ·1994-04-00 ·Pages 47-53

Tornero P, Conejero V, Vera P

Abstract

A Lycopersicon esculentum cDNA clone encoding an acidic-type pathogenesis-related protein (PR-1a1) was isolated, sequenced and characterized. It contains an open reading frame of 175 amino acids and the mature protein, after cleavage of the 21 amino acid signals peptide, has a pI of 5.24. The protein shows highest homology (75% identity) with the basic pathogenesis-related prb-1b protein from tobacco. The PR-1a1 gene shows constitutive expression in roots from tomato plants. It is expressed in leaves and stems upon viroid infection, and appears to be induced by ethylene. Comparative studies of this gene and a related basic isoform of PR-1 indicate that the expression of these two members of the PR-1 gene family in tomato may be differentially regulated upon viroid infection.

Related Genes
PR-1a1
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern DNA Gene Expression Regulation Hydrogen-Ion Concentration Molecular Sequence Data Multigene Family Organophosphorus Compounds/pharmacology Plant Growth Regulators/pharmacology Plant Proteins/genetics Plant Viruses/genetics,physiology Sequence Homology, Amino Acid Vegetables/genetics,microbiology Viroids/genetics,physiology Virus Replication
Chemicals
Organophosphorus Compounds Plant Growth Regulators Plant Proteins pathogenesis-related proteins, plant DNA ethephon
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tornero P
Department of Biotechnology, Universidad Politécnica de Valencia, Spain.
Conejero V
Vera P
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283875
Published
1994-04-00
Pages
47-53
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X71592
External Links
PubMed source DOI: 10.1007/BF00283875
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