Abstract
1. We describe the cloning of the inward rectifier K+ channel IRK1 from genomic DNA of mouse; the gene is intronless. 2. The IRK1 gene can be stably expressed in murine erythroleukaemia (MEL) cells. Such transfected cells show inward rectification under whole-cell recording. 3. Channels encoded by the IRK1 gene have an intrinsic gating that depends on voltage and [K+]o. Rate constants are reduced e-fold as the driving force on K+(V-EK) is reduced by 24.1 mV. 4. Removal of intracellular Mg2+ permits brief outward currents under depolarization. The instantaneous current-voltage relation may be fitted by an appropriate constant field expression. 5. Removal of intracellular Mg2+ speeds channel closure at positive voltages. In nominally zero [Mg2+]i, rate constants for the opening and closing of channels, processes which are first order, are similar to those of native channels.
MeSH Terms
Animals
Base Sequence
Cells, Cultured
Cloning, Molecular
Electrophysiology
Genome
Introns
Ion Channel Gating/physiology
Leukemia, Erythroblastic, Acute/metabolism
Magnesium/physiology
Mice
Molecular Sequence Data
Polymerase Chain Reaction
Potassium/physiology
Potassium Channels/drug effects,metabolism
Chemicals
Potassium Channels
Magnesium
Potassium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Stanfield P R
Department of Cell Physiology and Pharmacology, University of Leicester.
Davies N W
Shelton P A
Khan I A
Brammar W J
Standen N B
Conley E C
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