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PMID: 8188588 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequencing, chromosomal inactivation, and functional expression in Escherichia coli of ppsR, a gene which represses carotenoid and bacteriochlorophyll synthesis in Rhodobacter sphaeroides.

Journal of bacteriology ·Vol. 176 ·No. 10 ·1994-05-00 ·Pages 2869-76

Penfold RJ, Pemberton JM

Abstract

Sequencing of a DNA fragment that causes trans suppression of bacteriochlorophyll and carotenoid levels in Rhodobacter sphaeroides revealed two genes: orf-192 and ppsR. The ppsR gene alone is sufficient for photopigment suppression. Inactivation of the R. sphaeroides chromosomal copy of ppsR results in overproduction of both bacteriochlorophyll and carotenoid pigments. The deduced 464-amino-acid protein product of ppsR is homologous to the CrtJ protein of Rhodobacter capsulatus and contains a helix-turn-helix domain that is found in various DNA-binding proteins. Removal of the helix-turn-helix domain renders PpsR nonfunctional. The promoter of ppsR is located within the coding region of the upstream orf-192 gene. When this promoter is replaced by a lacZ promoter, ppsR is expressed in Escherichia coli. An R. sphaeroides DNA fragment carrying crtD', -E, and -F and bchC, -X, -Y, and -Z' exhibited putative promoter activity in E. coli. This putative promoter activity could be suppressed by PpsR in both E. coli and R. sphaeroides. These results suggest that PpsR is a transcriptional repressor. It could potentially act by binding to a putative regulatory palindrome found in the 5' flanking regions of a number of R. sphaeroides and R. capsulatus photosynthesis genes.

MeSH Terms
Aerobiosis Amino Acid Sequence Bacterial Proteins Bacteriochlorophylls/biosynthesis Base Sequence Carotenoids/biosynthesis DNA Mutational Analysis DNA-Binding Proteins/biosynthesis,genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Molecular Sequence Data Multigene Family/genetics Promoter Regions, Genetic/genetics Recombinant Proteins/biosynthesis Repressor Proteins/biosynthesis,genetics Restriction Mapping Rhodobacter sphaeroides/genetics Sequence Analysis, DNA Sequence Deletion Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Bacteriochlorophylls DNA-Binding Proteins Recombinant Proteins Repressor Proteins Carotenoids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Penfold R J
Microbiology Department, University of Queensland, St. Lucia, Brisbane, Australia.
Pemberton J M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.10.2869-2876.1994
Published
1994-05-00
Pages
2869-76
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205441
Subset
IM
Databases
GENBANK
L19596
External Links
PubMed source DOI: 10.1128/jb.176.10.2869-2876.1994 PMC Full Text View journal details
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