Mammalian gene expression is improved by use of a longer SV40 early polyadenylation cassette.

Nucleic acids research ·Vol. 21 ·No. 21 ·1993-10-25 ·Pages 4987-8

van den Hoff MJ, Labruyère WT, Moorman AF, Lamers WH

Abstract

暂无摘要

MeSH Terms

3T3 Cells Animals Gene Expression Genes, Reporter Genetic Vectors Mice Poly A/genetics Rats Simian virus 40/genetics Tumor Cells, Cultured

Chemicals

Poly A

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

van den Hoff M J

Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.

Labruyère W T

Moorman A F

Lamers W H

References (7)

7 references, click to expand

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
Rat carbamyl-phosphate synthetase I gene. Promoter sequence and tissue-specific transcriptional regulation in vitro.
J Biol Chem. 1987 Aug 5;262(22):10415-8 PMID: 3038878
A simple phase-extraction assay for chloramphenicol acyltransferase activity.
Gene. 1988 Jul 30;67(2):271-7 PMID: 3169576
Poly(A) signals.
Cell. 1991 Feb 22;64(4):671-4 PMID: 1671760
Optimized use of the firefly luciferase assay as a reporter gene in mammalian cell lines.
Biotechniques. 1989 Nov-Dec;7(10):1116-22 PMID: 2698191
The osmolarity of the electroporation medium affects the transient expression of genes.
Nucleic Acids Res. 1990 Nov 11;18(21):6464 PMID: 2173834
Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences.
Mol Cell Biol. 1989 Oct;9(10):4248-58 PMID: 2573828

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1993-10-25

Pages

4987-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311420

Subset

External Links

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