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PMID: 8177737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A base-paired hairpin structure essential for the functional priming signal for DNA replication of the broad host range plasmid RSF1010.

Nucleic acids research ·Vol. 21 ·No. 21 ·1993-10-25 ·Pages 4900-3

Miao DM, Honda Y, Tanaka K, Higashi A, Nakamura T, Taguchi Y, Sakai H, Komano T, Bagdasarian M

Abstract

The two single-strand DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010) of the broad host-range plasmid RSF1010 contain proposed stem-loop structures. Nine single base-change mutations in the stem of the ssiA structure, each of which destroyed a relevant base pairing, damaged the ssiA activity. A second single-base change was introduced into each of the nine ssiA mutants in such a way that the base pairing was restored. Only three out of nine second base changes that restored the base pairing restored the ssiA activity up to the wild-type level. Thus, the three are intramolecular suppressors. The results strongly suggested that, in the area of the stem of ssiA where the suppressor mutations fell, base pairing was the most important structural parameter for the ssiA activity. By contrast, it is most probable that, in the other part of the stem of ssiA, both base-pairing and the intrinsic base sequence were the major determinants of the ssiA activity.

Related Genes
ssiA ssiB
MeSH Terms
Base Sequence DNA Replication DNA, Bacterial/biosynthesis,chemistry DNA, Recombinant/biosynthesis,chemistry Genes, Suppressor Molecular Sequence Data Mutation Nucleic Acid Conformation R Factors
Chemicals
DNA, Bacterial DNA, Recombinant
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Miao D M
Department of Agricultural Chemistry, Kyoto University, Japan.
Honda Y
Tanaka K
Higashi A
Nakamura T
Taguchi Y
Sakai H
Komano T
Bagdasarian M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.21.4900
Published
1993-10-25
Pages
4900-3
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311403
Subset
IM
Databases
GENBANK
M28829
External Links
PubMed source DOI: 10.1093/nar/21.21.4900 PMC Full Text View journal details
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