Surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum.

British journal of cancer ·Vol. 38 ·No. 3 ·1978-09-00 ·Pages 456-60

Embleton MJ, Iype PT

Abstract

Cultured rat liver cells induced a strong antibody response in syngeneic rats, directed against foetal calf serum components which were incorporated into the liver cell surface from the cell-culture medium. This antibody could be removed by absorption with liver cells or glutaraldehyde-fixed foetal calf serum. It is possible that the antigenic cross-reactivity observed in earlier studies with cultured cells treated with carcinogens could be due to this foetal calf serum component.

MeSH Terms

Animals Antibody Formation Antigens, Surface/analysis Cells, Cultured Culture Media Epithelium/immunology Fetal Blood Liver/immunology Male Rats Rats, Inbred Strains alpha-Fetoproteins

Chemicals

Antigens, Surface Culture Media alpha-Fetoproteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Embleton M J

Iype P T

References (14)

14 references, click to expand

Properties and malignant transformation of established rat liver parenchymal cells in culture.
J Natl Cancer Inst. 1975 Aug;55(2):375-84 PMID: 1159824
Immunologic identification of fetal calf serum-derived proteins on the surfaces of cultured transformed and untransformed rat cells.
Int J Cancer. 1977 Nov 15;20(5):798-804 PMID: 200575
Antigenic diversity of tumors chemically induced within the progeny of a single cell.
Int J Cancer. 1972 Jul 15;10(1):1-8 PMID: 4122455
Cultures from adult rat liver cells. I. Establishment of monolayer cell-cultures from normal liver.
J Cell Physiol. 1971 Oct;78(2):281-8 PMID: 4333457
Demonstration of cell-surface antigens on chemically induced tumors.
Ann N Y Acad Sci. 1971 Jun 21;177:268-78 PMID: 4333751
Antigenicity of clones of mouse prostate cells transformed in vitro.
Int J Cancer. 1972 Jan 15;9(1):8-18 PMID: 4552543
Cell surface antigenic changes induced in normal adult rat liver cells by carcinogen treatment in vitro.
Br J Cancer. 1973 Feb;27(2):128-33 PMID: 4571664
Carcinoma after malignant conversion in vitro of epithelial-like cells from rat liver following exposure to chemical carcinogens.
Cancer Res. 1973 Mar;33(3):606-12 PMID: 4689906
Malignant transformation in vitro of rat liver cells by dimethylnitrosamine and N-methyl-N'-nitro-N-nitrosoguanidine.
Br J Cancer. 1973 Sep;28(3):215-20 PMID: 4743905
Natural antibody in human serum to a neoantigen in human cultured cells grown in fetal bovine serum.
J Natl Cancer Inst. 1974 Apr;52(4):1051-8 PMID: 4857031
Antigenicity of cells derived from mouse prostate cells after malignant transformation in vitro by carcinogenic hydrocarbons.
Cancer Res. 1970 Jun;30(6):1593-7 PMID: 5457933
Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells.
Cancer Res. 1978 Mar;38(3):624-34 PMID: 626967
Tumor-specific antigens on rat liver cells transformed in vitro by chemical carcinogens.
J Natl Cancer Inst. 1978 Jan;60(1):125-9 PMID: 75266
Neoantigens on chemically transformed cloned C3H mouse embryo cells.
Cancer Res. 1975 Aug;35(8):2049-55 PMID: 807321

Article Info

Journal

British journal of cancer

Abbr.

Br J Cancer

ISSN

0007-0920

DOI

10.1038/bjc.1978.229

Published

1978-09-00

Pages

456-60

Language

English

Region

England

NLM ID

0370635

PMCID

PMC2009748

Subset

External Links

PubMed source DOI: 10.1038/bjc.1978.229 PMC Full Text View journal details

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Surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum.

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