Home LiteratureArticle Details
PMID: 8160768 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunohistochemical detection of alpha-catenin expression in human cancers.

The American journal of pathology ·Vol. 144 ·No. 4 ·1994-04-00 ·Pages 667-74

Shiozaki H, Iihara K, Oka H, Kadowaki T, Matsui S, Gofuku J, Inoue M, Nagafuchi A, Tsukita S, Mori T

Abstract

The function of E-cadherin is thought to be regulated by its associated cytoplasmic proteins including alpha-catenin. To determine whether possible downregulation of alpha-catenin expression may play a role in tumor invasion and metastasis through the dysfunction of E-cadherin, we investigated the expression of alpha-catenin in human carcinoma samples (esophagus, stomach, and colon) by immunohistochemistry using our monoclonal antibody against alpha-catenin (alpha-18). Normal epithelium expressed alpha-catenin strongly without exception. However, alpha-catenin expression was frequently reduced in primary tumors of esophagus (12 of 15:80%), stomach (14 of 20: 70%), and colon (8 of 10: 80%). Of the tumors with reduced alpha-catenin expression, alpha-catenin expression was completely negative in 70.6% of them (9 of 12 in esophagus, 9 of 14 in stomach, and 6 of 8 in colon). These results also suggested that some human cancer cells may have impaired E-cadherin-mediated cell adhesiveness through the downregulation of alpha-catenin expression.

MeSH Terms
Adenocarcinoma/metabolism,pathology Adult Aged Cadherins/metabolism Carcinoma, Squamous Cell/metabolism,pathology Colonic Neoplasms/metabolism,pathology Cytoskeletal Proteins/metabolism Down-Regulation Esophageal Neoplasms/metabolism,pathology Female Humans Immunoenzyme Techniques Infant Male Middle Aged Neoplasm Staging Stomach Neoplasms/metabolism,pathology alpha Catenin
Chemicals
CTNNA1 protein, human Cadherins Cytoskeletal Proteins alpha Catenin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Shiozaki H
Department of Surgery II, Osaka University Medical School, Japan.
Iihara K
Oka H
Kadowaki T
Matsui S
Gofuku J
Inoue M
Nagafuchi A
Tsukita S
Mori T
References (28)
28 references, click to expand
  1. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
    J Histochem Cytochem. 1981 Apr;29(4):577-80 PMID: 6166661
  2. Reduction of E-cadherin levels and deletion of the alpha-catenin gene in human prostate cancer cells.
    Cancer Res. 1993 Aug 1;53(15):3585-90 PMID: 8339265
  3. Expressed recombinant cadherins mediate cell sorting in model systems.
    Cell. 1988 Sep 23;54(7):993-1001 PMID: 3416359
  4. Cell binding function of E-cadherin is regulated by the cytoplasmic domain.
    EMBO J. 1988 Dec 1;7(12):3679-84 PMID: 3061804
  5. Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion.
    J Cell Biol. 1989 Jun;108(6):2435-47 PMID: 2661563
  6. Unstable expression of E-cadherin adhesion molecules in metastatic ovarian tumor cells.
    Jpn J Cancer Res. 1989 May;80(5):459-63 PMID: 2502522
  7. The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.
    EMBO J. 1989 Jun;8(6):1711-7 PMID: 2788574
  8. Cadherin-mediated specific cell adhesion and animal morphogenesis.
    Ciba Found Symp. 1989;144:243-9; discussion 250-4, 290-5 PMID: 2673680
  9. Cell sorting-out is modulated by both the specificity and amount of different cell adhesion molecules (CAMs) expressed on cell surfaces.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):7043-7 PMID: 2780560
  10. Uvomorulin-catenin complex: cytoplasmic anchorage of a Ca2+-dependent cell adhesion molecule.
    Bioessays. 1989 Oct;11(4):88-91 PMID: 2695079
  11. Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule.
    Proc Natl Acad Sci U S A. 1990 Jun;87(11):4246-50 PMID: 2349235
  12. Cadherin cell adhesion receptors as a morphogenetic regulator.
    Science. 1991 Mar 22;251(5000):1451-5 PMID: 2006419
  13. The 102 kd cadherin-associated protein: similarity to vinculin and posttranscriptional regulation of expression.
    Cell. 1991 May 31;65(5):849-57 PMID: 1904011
  14. Expression of immunoreactive E-cadherin adhesion molecules in human cancers.
    Am J Pathol. 1991 Jul;139(1):17-23 PMID: 1713020
  15. The uvomorulin-anchorage protein alpha catenin is a vinculin homologue.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9156-60 PMID: 1924379
  16. E-cadherin expression in squamous cell carcinomas of head and neck: inverse correlation with tumor dedifferentiation and lymph node metastasis.
    Cancer Res. 1991 Dec 1;51(23 Pt 1):6328-37 PMID: 1933895
  17. Regulation of embryonic cell adhesion by the cadherin cytoplasmic domain.
    Cell. 1992 Apr 17;69(2):225-36 PMID: 1568244
  18. Expression of E-cadherin in normal, benign, and malignant tissues of female genital organs.
    Am J Clin Pathol. 1992 Jul;98(1):76-80 PMID: 1615930
  19. Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization.
    Cell. 1992 Jul 24;70(2):293-301 PMID: 1638632
  20. Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
    J Cell Biol. 1992 Aug;118(3):671-9 PMID: 1639850
  21. Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastatic fibroblasts.
    J Cell Biol. 1992 Aug;118(3):703-14 PMID: 1639852
  22. Immunohistochemical evaluation of E-cadherin adhesion molecule expression in human gastric cancer.
    Virchows Arch A Pathol Anat Histopathol. 1992;421(2):149-56 PMID: 1514246
  23. Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer.
    Cancer Res. 1992 Sep 15;52(18):5104-9 PMID: 1516067
  24. Cadherin dysfunction in a human cancer cell line: possible involvement of loss of alpha-catenin expression in reduced cell-cell adhesiveness.
    Cancer Res. 1992 Oct 15;52(20):5770-4 PMID: 1394201
  25. Molecular linkage between cadherins and actin filaments in cell-cell adherens junctions.
    Curr Opin Cell Biol. 1992 Oct;4(5):834-9 PMID: 1419062
  26. Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.
    J Cell Biol. 1993 Feb;120(3):757-66 PMID: 8425900
  27. Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis.
    Cancer Res. 1993 Apr 1;53(7):1696-701 PMID: 8453644
  28. Some structural and functional aspects of the cell adhesion molecule uvomorulin.
    Cell Differ. 1984 Dec;15(2-4):269-73 PMID: 6336035
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1994-04-00
Pages
667-74
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1887239
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2