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PMID: 8146161 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heme oxygenase 1 mediates an adaptive response to oxidative stress in human skin fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 7 ·1994-03-29 ·Pages 2607-10

Vile GF, Basu-Modak S, Waltner C, Tyrrell RM

Abstract

Oxidative stress of human skin fibroblasts by treatment with ultraviolet A (UVA) radiation has been shown to lead to an increase in levels of the heme catabolizing enzyme heme oxygenase 1 [heme, hydrogen-donor:oxygen oxidoreductase (alpha-methene-oxidizing, hydroxylating), EC 1.14.99.3] and the iron storage protein ferritin. Here we show that human skin fibroblasts, preirradiated with UVA, sustain less membrane damage during a subsequent exposure to UVA radiation than cells that had not been preirradiated. Pretreating cells with heme oxygenase 1 antisense oligonucleotide inhibited the irradiation-dependent induction of both the heme oxygenase I enzyme and ferritin and abolished the protective effect of preirradiation. Inhibition of the UVA preirradiation-dependent increase in ferritin, but not heme oxygenase, with desferrioxamine also abolished the protection. This identifies heme oxygenase 1 as a crucial enzymatic intermediate in an oxidant stress-inducible antioxidant defense mechanism, involving ferritin, in human skin fibroblasts.

MeSH Terms

Adaptation, Physiological Antioxidants/metabolism Cells, Cultured Deferoxamine/pharmacology Enzyme Induction/drug effects,radiation effects Ferritins/biosynthesis Fibroblasts/enzymology,radiation effects Heme Oxygenase (Decyclizing)/metabolism Humans Lipid Peroxidation/drug effects,radiation effects Oligonucleotides, Antisense/pharmacology Oxidation-Reduction Skin/enzymology,radiation effects Ultraviolet Rays

Chemicals

Antioxidants Oligonucleotides, Antisense Ferritins Heme Oxygenase (Decyclizing) Deferoxamine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Vile G F

Swiss Institute for Experimental Cancer Research, ISREC, Lausanne.

Basu-Modak S

Waltner C

Tyrrell R M

References (20)

20 references, click to expand

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Membrane damage induced in cultured human skin fibroblasts by UVA irradiation.
Photochem Photobiol. 1993 Oct;58(4):515-20 PMID: 8248324
Increase in the synthesis of a Mr 32,000 protein in BALB/c 3T3 cells after treatment with tumor promoters, chemical carcinogens, metal salts, and heat shock.
Cancer Res. 1986 May;46(5):2474-81 PMID: 3754488
Quantitative differences in host cell reactivation of ultraviolet-damaged virus in human skin fibroblasts and epidermal keratinocytes cultured from the same foreskin biopsy.
Cancer Res. 1986 Jun;46(6):2665-9 PMID: 2421882
Induction of 32- and 34-kDa stress proteins by sodium arsenite, heavy metals, and thiol-reactive agents.
J Biol Chem. 1986 Oct 5;261(28):13381-6 PMID: 3093479
Both near ultraviolet radiation and the oxidizing agent hydrogen peroxide induce a 32-kDa stress protein in normal human skin fibroblasts.
J Biol Chem. 1987 Oct 25;262(30):14821-5 PMID: 3667606
Human heme oxygenase cDNA and induction of its mRNA by hemin.
Eur J Biochem. 1988 Feb 1;171(3):457-61 PMID: 3345742
A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity.
J Immunol Methods. 1988 Nov 25;115(1):61-9 PMID: 3192948
Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite.
Proc Natl Acad Sci U S A. 1989 Jan;86(1):99-103 PMID: 2911585
The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase.
FEBS Lett. 1989 Mar 13;245(1-2):173-6 PMID: 2924920
Induction of haem oxygenase as a defence against oxidative stress.
Free Radic Res Commun. 1990;9(2):101-12 PMID: 2189794
Induction of heme oxygenase: a general response to oxidant stress in cultured mammalian cells.
Cancer Res. 1991 Feb 1;51(3):974-8 PMID: 1988141
Regulation of ferritin and heme oxygenase synthesis in rat fibroblasts by different forms of iron.
Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):688-92 PMID: 1992460
Exposure of endothelial cells to free heme potentiates damage mediated by granulocytes and toxic oxygen species.
Lab Invest. 1991 May;64(5):648-55 PMID: 2030579
Heme oxygenase induction by CoCl2, Co-protoporphyrin IX, phenylhydrazine, and diamide: evidence for oxidative stress involvement.
Arch Biochem Biophys. 1991 May 1;286(2):610-7 PMID: 1716866
Induction of heme oxygenase is a rapid, protective response in rhabdomyolysis in the rat.
J Clin Invest. 1992 Jul;90(1):267-70 PMID: 1634613
Ferritin: a cytoprotective antioxidant strategem of endothelium.
J Biol Chem. 1992 Sep 5;267(25):18148-53 PMID: 1517245
Variable glutathione levels and expression of antioxidant enzymes in human endothelial cells.
Am J Physiol. 1993 May;264(5 Pt 1):L482-9 PMID: 8498525
Oxidative stress resulting from ultraviolet A irradiation of human skin fibroblasts leads to a heme oxygenase-dependent increase in ferritin.
J Biol Chem. 1993 Jul 15;268(20):14678-81 PMID: 8325845
Extraction of tissue lipids with a solvent of low toxicity.
Methods Enzymol. 1981;72:5-7 PMID: 7311848

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.91.7.2607

Published

1994-03-29

Pages

2607-10

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC43418

Subset

External Links

PubMed source DOI: 10.1073/pnas.91.7.2607 PMC Full Text View journal details

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Heme oxygenase 1 mediates an adaptive response to oxidative stress in human skin fibroblasts.

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